Native Human Immunoglobulin G (IgG), Fab'2 Protein

Name: Native Human Immunoglobulin G (IgG), Fab'2 Protein
Brand: Beta LifeScience
SKU: BAR-0082P
Price: 375.0 USD
Availability: InStock

Collections: All products, Christmas & new year research sale — 30% off storewide, Native proteins

Price

Size

1mg

Quantity

Submit an inquiry or email sales for a custom bulk quote. Our products are highly customizable to meet your specific needs. You can choose options such as endotoxin removal, liquid or lyophilized forms, preferred tags, and the desired functional sequence range for proteins. Submitting a written inquiry expedites the quoting process.

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Product Overview

Description	F(ab’)2 is bivalent in relation to the antigen binding sites. It lacks the Fc region of the IgG molecule, making it a valuable tool in antibody studies in which binding of the Fc region is not desireable. In addition, when working with low affinity antibodies, bivalent F(ab’)2 is preferable to univalent Fab or Fab’ fragments given its higher association constant.
Source	Human Plasma
Form	Frozen
Buffer	Frozen in 20 mM sodium phosphate, pH 7.4, with 150 mM NaCl and 0.05% NaN₃
Purity	Greater than 95% as determined by SDS-PAGE. Single arc by IEP against antisera to whole human serum.
Molecular Weight	110 kDa
Protein Determination	Extinction Coefficient (E) = 1.36 (0.1% at 280 nm, 1 cm pathway)
Storage/Handling	≤ -20°C

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.