Native Human ApoCeruloplasmin

Name: Native Human ApoCeruloplasmin
Brand: Beta LifeScience
SKU: BAR-0052P
Availability: InStock

Collections: All products, Native proteins

Our products are highly customizable to meet your specific needs. You can choose options such as endotoxin removal, liquid or lyophilized forms, preferred tags, and the desired functional sequence range for proteins. Submitting a written inquiry expedites the quoting process.

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Product Overview

Description	Native Human ApoCeruloplasmin represents the iron-free form of the major copper-carrying glycoprotein in plasma, serving as an essential molecular precursor that undergoes copper incorporation to form functional holoceruloplasmin with its characteristic ferroxidase activity. This metal-free glycoprotein maintains the structural framework for binding six copper atoms while exhibiting dynamic interactions with cellular copper transporters during hepatic ceruloplasmin biosynthesis, with its stability and conformation directly influencing the efficiency of copper loading and subsequent secretion into circulation. Beyond its copper transport role, the apoprotein form participates in regulatory mechanisms for metal homeostasis, demonstrates altered kinetics in Wilson's disease pathogenesis where copper incorporation fails, and provides a crucial experimental tool for studying metalloprotein assembly while retaining certain antioxidant properties through copper-independent radical scavenging capabilities.
Source	Human Plasma
Form	Lyophilized Powder
Buffer	Lyophilized from 0.1M NaCl + 15 mM NaN3
Purity	Greater than 95% as determined by SDS-PAGE.
Molecular Weight	132kDa
Protein Determination	Extinction Coefficient (E) = 1.50 (0.1% at 280 nm, 1 cm pathway)
Storage/Handling	≤ -20 °C

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.