MagicScript II RT Master Mix for qPCR

Name: MagicScript II RT Master Mix for qPCR
Brand: BetaLifeSci
SKU: ENY-102
Availability: InStock

Collections: Featured enzyme molecules, Tool enzymes & other reagents

Our products are highly customizable to meet your specific needs. You can choose options such as endotoxin removal, liquid or lyophilized forms, preferred tags, and the desired functional sequence range for proteins. Submitting a written inquiry expedites the quoting process.

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Product Overview

Description	MagicScript II Reverse Transcriptase is a genetically engineered MMLV reverse transcriptase (RT) with reduced RNase H activity and increased thermal stability compared to wild-type MMLV RT. MagicScript II RT Master Mix for qPCR is a reverse transcription detection reagent suitable for two-step RT-qPCR. The 5X MagicScript II RT Mix contains all the reagents required for Reverse transcription reactions [MagicScript II Reverse Transcriptase, dNTP, RNase Inhibitor, Random primers/Oligo (dT)23VN Primer Mix and optimized reaction solution]. The volume of RNA template can be up to 80% of the total volume, which is very suitable for the reverse transcription reaction of low concentration RNA template. 5 x MagicScript II RT Mix will not freeze at -20°C storage, which is very convenient to use.
Applications	cDNA Synthesis; RT-PCR & cDNA Synthesis; Reverse Transcription (cDNA Synthesis)
Size	10 rxn / 100 rxn
Concentration	5x
Components	Nuclease-free H2O; 5X MagicScript II RT Mix
Usage	For Research Use Only
Storage	Store the MagicScript II RT Master Mix for qPCR at -20°C. Please avoid repeated freeze-thaw cycles.

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.