ILK Recombinant Monoclonal Antibody

Name: ILK Recombinant Monoclonal Antibody
Brand: Beta LifeScience
SKU: BLC-06715A
Price: 299.0 USD
Availability: InStock

Immunofluorescence staining of MCF7 Cells with ILK Recombinant Monoclonal Antibody at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4℃. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).

Immunoprecipitating ILK in Hela whole cell lysate | Lane 1: Rabbit control IgG instead of ILK Recombinant Monoclonal Antibody in Hela whole cell lysate. | For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) | Lane 2: ILK Recombinant Monoclonal Antibody(2µg)+ Hela whole cell lysate(500µg) | Lane 3: Hela whole cell lysate (10µg)

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Product Overview

Product Title	ILK Recombinant Monoclonal Antibody
Description	The recombinant antibody against ILK was produced using a synthesized peptide derived from human ILK as the immunogen. This antibody exists as a non-conjugated isotype Rabbit IgG, Affinity-chromatography purified. This antibody has been validated on ELISA, WB, IF, IP.
Uniprot Id - Alink	Q13418
Reactivity	Human, Mouse
Target Name	ILK
Target Synonyms	Integrin-linked protein kinase (EC 2.7.11.1) (59 kDa serine/threonine-protein kinase) (ILK-1) (ILK-2) (p59ILK), ILK, ILK1 ILK2
Immunogen Description	A synthesized peptide derived from human ILK
Immunogen Species	Human
Immunogen Sequence	Complete sequences for the immunogen, target protein, and peptides are available upon request.
Conjugate	Non-conjugated
Clonality	Monoclonal
Isotype	Rabbit IgG
Purification Method	Affinity-chromatography purified
Buffer	Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form	Liquid
Application	ELISA, WB, IF, IP
Storage Conditions	Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.