Rabbit anti-Human VPS35 Polyclonal Antibody

Beta LifeScience SKU/CAT #: BLC-03802A
The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using the antibody Antibody) at dilution 1/30, on the right is treated with fusion protein. (Original magnification: ×200)
The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using the antibody Antibody) at dilution 1/30, on the right is treated with fusion protein. (Original magnification: ×200)
The image on the left is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using VPS35 Antibody(VPS35 Antibody) at dilution 1/30, on the right is treated with fusion protein. (Original magnification: ×200)
The image on the left is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using VPS35 Antibody(VPS35 Antibody) at dilution 1/30, on the right is treated with fusion protein. (Original magnification: ×200)
Gel: 6%SDS-PAGE, Lysate: 40 μg, Lane: Human fetal brain tissue, Primary antibody: VPS35 Antibody(VPS35 Antibody) at dilution 1/250 dilution, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 20 seconds
Gel: 6%SDS-PAGE, Lysate: 40 μg, Lane: Human fetal brain tissue, Primary antibody: VPS35 Antibody(VPS35 Antibody) at dilution 1/250 dilution, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 20 seconds

Rabbit anti-Human VPS35 Polyclonal Antibody

Beta LifeScience SKU/CAT #: BLC-03802A
Our products are highly customizable to meet your specific needs. You can choose options such as endotoxin removal, liquid or lyophilized forms, preferred tags, and the desired functional sequence range for proteins. Submitting a written inquiry expedites the quoting process.

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Product Overview

Product Title Rabbit anti-Human VPS35 Polyclonal Antibody
Description The antibody against VPS35 was raised in rabbit using the Fusion protein of Human VPS35 as the immunogen. This antibody exists as a non-conjugated isotype IgG, Antigen affinity purified. This antibody has been validated on ELISA, WB, IHC.
Uniprot Id - Alink Q96QK1
Host Species Rabbit
Reactivity Human, Mouse
Target Name VPS35
Target Synonyms DKFZp434E1211 antibody; DKFZp434P1672 antibody; FLJ10752 antibody; FLJ13588 antibody; FLJ20388 antibody; hVPS35 antibody; Maternal embryonic 3 antibody; Maternal-embryonic 3 antibody; MEM 3 antibody; MEM3 antibody; PARK17 antibody; TCCCTA00141 antibody; Vacuolar protein sorting 35 (yeast) antibody; Vacuolar protein sorting 35 antibody; Vacuolar protein sorting 35 homolog antibody; Vacuolar protein sorting associated protein 35 antibody; Vacuolar protein sorting-associated protein 35 antibody; Vesicle protein sorting 35 antibody; VPS 35 antibody; VPS35 antibody; VPS35_HUMAN antibody
Immunogen Description Fusion protein of Human VPS35
Immunogen Species Human
Immunogen Sequence Complete sequences for the immunogen, target protein, and peptides are available upon request.
Conjugate Non-conjugated
Clonality Polyclonal
Isotype IgG
Purification Method Antigen affinity purified
Buffer pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Form Liquid
Application ELISA, WB, IHC
Storage Conditions Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.

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