Rabbit anti-Human SLC12A2 Polyclonal Antibody

Beta LifeScience SKU/CAT #: BLC-00777A
Western Blot Positive WB detected in: Hela whole cell lysate,A549 whole cell lysate,JK whole cell lysate All lanes: SLC12A2 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 132 kDa Observed band size: 132 kDa
Western Blot Positive WB detected in: Hela whole cell lysate,A549 whole cell lysate,JK whole cell lysate All lanes: SLC12A2 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 132 kDa Observed band size: 132 kDa
Immunofluorescence staining of Hela cell with SLC12A2 Antibody at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunofluorescence staining of Hela cell with SLC12A2 Antibody at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunofluorescence staining of Hela cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunofluorescence staining of Hela cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Western Blot Positive WB detected in: Hela whole cell lysate,A549 whole cell lysate,JK whole cell lysate All lanes: SLC12A2 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 132 kDa Observed band size: 132 kDa
Immunofluorescence staining of Hela cell with SLC12A2 Antibody at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunofluorescence staining of Hela cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Rabbit anti-Human SLC12A2 Polyclonal Antibody

Beta LifeScience SKU/CAT #: BLC-00777A
Our products are highly customizable to meet your specific needs. You can choose options such as endotoxin removal, liquid or lyophilized forms, preferred tags, and the desired functional sequence range for proteins. Submitting a written inquiry expedites the quoting process.

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Product Overview

Product Title Rabbit anti-Human SLC12A2 Polyclonal Antibody
Description The antibody against SLC12A2 was raised in rabbit using the Recombinant Human Solute carrier family 12 member 2 protein as the immunogen. This antibody exists as a non-conjugated isotype IgG, Antigen affinity purified. This antibody has been validated on ELISA, WB, IF.
Uniprot Id - Alink P55011
Host Species Rabbit
Reactivity Human
Target Name SLC12A2
Target Synonyms Basolateral Na-K-Cl symporter antibody; BSC antibody; BSC2 antibody; Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 1 antibody; mBSC2 antibody; MGC104233 antibody; NKCC1 antibody; PPP1R141 antibody; Protein phosphatase 1; regulatory subunit 141 antibody; S12A2_HUMAN antibody; Slc12a2 antibody; sodium-potassium-chloride cotransporter 1 antibody; Solute carrier family 12 (sodium/potassium/chloride transporter); member 2 antibody; solute carrier family 12 (sodium/potassium/chloride transporters) member 2 antibody; Solute carrier family 12 member 2 antibody; sy-ns antibody
Immunogen Description Recombinant Human Solute carrier family 12 member 2 protein
Immunogen Species Human
Immunogen Sequence Complete sequences for the immunogen, target protein, and peptides are available upon request.
Conjugate Non-conjugated
Clonality Polyclonal
Isotype IgG
Purification Method Antigen affinity purified
Buffer 0.02% sodium azideConstituents:PBS containing 50% glycerol
Form Liquid
Application ELISA, WB, IF
Storage Conditions Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.

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