Rabbit anti-Human RNF40 Polyclonal Antibody

Name: Rabbit anti-Human RNF40 Polyclonal Antibody
Brand: Beta LifeScience
SKU: BLC-03888A
Price: 299.0 USD
Availability: InStock

The image on the left is immunohistochemistry of paraffin-embedded Human breast cancer tissue using the antibody Antibody) at dilution 1/25, on the right is treated with fusion protein. (Original magnification: ×200)

The image on the left is immunohistochemistry of paraffin-embedded Human brain tissue using RNF40 Antibody(RNF40 Antibody) at dilution 1/25, on the right is treated with fusion protein. (Original magnification: ×200)

Gel: 6%SDS-PAGE, Lysate: 40 μg, Lane 1-3: Hela cells, 231 cells, A172 cells, Primary antibody: RNF40 Antibody(RNF40 Antibody) at dilution 1/400, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 minute

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Our products are highly customizable to meet your specific needs. You can choose options such as endotoxin removal, liquid or lyophilized forms, preferred tags, and the desired functional sequence range for proteins. Submitting a written inquiry expedites the quoting process.

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Product Overview

Product Title	Rabbit anti-Human RNF40 Polyclonal Antibody
Description	The antibody against RNF40 was raised in rabbit using the Fusion protein of Human RNF40 as the immunogen. This antibody exists as a non-conjugated isotype IgG, Antigen affinity purified. This antibody has been validated on ELISA, WB, IHC.
Uniprot Id - Alink	O75150
Host Species	Rabbit
Reactivity	Human, Mouse, Rat
Target Name	RNF40
Target Synonyms	95 kDa retinoblastoma associated protein antibody; 95 kDa retinoblastoma protein binding protein antibody; 95 kDa retinoblastoma-associated protein antibody; BRE 1B antibody; BRE1 B antibody; BRE1 E3 ubiquitin ligase homolog B antibody; BRE1-B antibody; BRE1B antibody; BRE1B_HUMAN antibody; DKFZp686K191 antibody; E3 ubiquitin protein ligase BRE1B antibody; E3 ubiquitin-protein ligase BRE1B antibody; KIAA0661 antibody; MGC13051 antibody; Rb associated protein antibody; RBP 95 antibody; RBP95 antibody; RING finger protein 40 antibody; Ring finger protein 40 E3 ubiquitin protein ligase antibody; RNF 40 antibody; Rnf40 antibody; STARING antibody
Immunogen Description	Fusion protein of Human RNF40
Immunogen Species	Human
Immunogen Sequence	Complete sequences for the immunogen, target protein, and peptides are available upon request.
Conjugate	Non-conjugated
Clonality	Polyclonal
Isotype	IgG
Purification Method	Antigen affinity purified
Buffer	pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Form	Liquid
Application	ELISA, WB, IHC
Storage Conditions	Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.