Rabbit anti-Human PRUNE1 Polyclonal Antibody
Beta LifeScience
SKU/CAT #: BLC-04922A

Western Blot | Positive WB detected in: HepG2 whole cell lysate, MCF-7 whole cell lysate, Jurkat whole cell lysate | All lanes: PRUNE1 antibody at 4µg/ml | Secondary | Goat polyclonal to rabbit IgG at 1/50000 dilution | Predicted band size: 51, 43, 31, 27, 25, 19 kDa | Observed band size: 60 kDa

IHC image of PRUNE1 Antibody diluted at 1:300 and staining in paraffin-embedded human lung tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunofluorescence staining of HepG2 cells with PRUNE1 Antibody at 1:135, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Rabbit anti-Human PRUNE1 Polyclonal Antibody
Beta LifeScience
SKU/CAT #: BLC-04922A
Our products are highly customizable to meet your specific needs. You can choose options such as endotoxin removal, liquid or lyophilized forms, preferred tags, and the desired functional sequence range for proteins. Submitting a written inquiry expedites the quoting process.
Product Overview
Product Title | Rabbit anti-Human PRUNE1 Polyclonal Antibody |
Description | The antibody against PRUNE1 was raised in rabbit using the Recombinant Human Exopolyphosphatase PRUNE1 protein (1-168AA) as the immunogen. This antibody exists as a non-conjugated isotype IgG, purified by protein G with a purity greater than 95%. This antibody has been validated on ELISA, WB, IHC, IF, IP. |
Uniprot Id - Alink | Q86TP1 |
Host Species | Rabbit |
Reactivity | Human |
Target Name | PRUNE1 |
Target Synonyms | DRES-17 antibody; DRES17 antibody; Drosophila-related expressed sequence 17 antibody; hPrune antibody; HTcD37 antibody; Protein prune homolog antibody; PRUNE antibody; Prune homolog antibody; PRUNE like protein antibody; PRUNE_HUMAN antibody; TcD37 homolog antibody |
Immunogen Description | Recombinant Human Exopolyphosphatase PRUNE1 protein (1-168AA) |
Immunogen Species | Human |
Immunogen Sequence | Complete sequences for the immunogen, target protein, and peptides are available upon request. |
Conjugate | Non-conjugated |
Clonality | Polyclonal |
Isotype | IgG |
Purification Method | >95%, Protein G purified |
Buffer | 0.03% Proclin 300Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
Form | Liquid |
Application | ELISA, WB, IHC, IF, IP |
Storage Conditions | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |