Mouse anti-Human PPA1 Monoclonal antibody

Name: Mouse anti-Human PPA1 Monoclonal antibody
Brand: Beta LifeScience
SKU: BLC-06403A
Price: 299.0 USD
Availability: InStock

Western Blot Positive WB detected in: PPA1 antibody at 1:1000 Lane 1: Hela whole cell lysate Lane 2: HepG2 whole cell lysate Lane 3: 293T whole cell lysate Lane 4: MCF-7 whole cell lysate Lane 5: U87 whole cell lysateSecondary Goat polyclonal to Mouse IgG at 1/20000 dilution Predicted band size: 33KDa Observed band size: 33 KDa Exposure time: 5min

Immunofluorescence staining of Hela cells with PPA1 Monoclonal Antibody at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).

Overlay Peak curve showing HepG2 cells stained with PPA1 Monoclonal Antibody (red line) at 1:100. The cells were incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1µg/1*106cells) for 1h at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1µg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.

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Product Overview

Product Title	Mouse anti-Human PPA1 Monoclonal antibody
Description	The antibody against PPA1 was raised in mouse using the Recombinant Human Inorganic pyrophosphatase protein (1-289AA) as the immunogen. This antibody exists as a non-conjugated isotype IgG1, purified by protein G with a purity greater than 95%. This antibody has been validated on ELISA, WB, IF, FC.
Uniprot Id - Alink	Q15181
Host Species	Mouse
Reactivity	Human
Target Name	PPA1
Target Synonyms	cytosolic inorganic pyrophosphatase antibody; diphosphate phosphohydrolase antibody; inorganic diphosphatase antibody; inorganic pyrophosphatase 1 antibody; Inorganic pyrophosphatase antibody; IOPPP antibody; IPYR_HUMAN antibody; PP antibody; PP1 antibody; PPA1 antibody; PPase antibody; Pyp antibody; pyrophosphatase, inorganic, 1 antibody; Pyrophosphate phospho hydrolase antibody; Pyrophosphate phospho-hydrolase antibody; SID6-8061 antibody
Immunogen Description	Recombinant Human Inorganic pyrophosphatase protein (1-289AA)
Immunogen Species	Human
Immunogen Sequence	Complete sequences for the immunogen, target protein, and peptides are available upon request.
Conjugate	Non-conjugated
Clonality	Monoclonal
Isotype	IgG1
Purification Method	>95%, Protein G purified
Buffer	0.03% Proclin 300Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Form	Liquid
Application	ELISA, WB, IF, FC
Storage Conditions	Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.