Rabbit anti-Human MAPK14 Polyclonal Antibody

Name: Rabbit anti-Human MAPK14 Polyclonal Antibody
Brand: Beta LifeScience
SKU: BLC-05569A
Price: 299.0 USD
Availability: InStock

Western blot analysis of extracts from Hela cells untreated or treated with UV for the indicated times, using P38 MAPK(Phospho-Tyr182) Antibody.

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using P38 MAPK(Phospho-Tyr182) Antibody(left) or the same antibody preincubated with blocking peptide(right).

Western blot analysis of extracts from Hela cells, treated with TNFa or calf intestinal phosphatase (CIP), using P38 MAPK (Phospho-Tyr182) Antibody.

Our products are highly customizable to meet your specific needs. You can choose options such as endotoxin removal, liquid or lyophilized forms, preferred tags, and the desired functional sequence range for proteins. Submitting a written inquiry expedites the quoting process.

Product Overview

Product Title	Rabbit anti-Human MAPK14 Polyclonal Antibody
Description	The antibody against MAPK14 was raised in rabbit using the Peptide sequence around phosphorylation site of tyrosine 182 (T-G-Y(p)-V-A) derived from Human P38 MAPK. as the immunogen. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi This antibody has been validated on ELISA, WB, IHC.
Uniprot Id - Alink	Q16539
Host Species	Rabbit
Reactivity	Human, Mouse, Rat
Target Name	MAPK14
Target Synonyms	CSAID Binding Protein 1 antibody; CSAID binding protein antibody; CSAID-binding protein antibody; Csaids binding protein antibody; CSBP 1 antibody; CSBP 2 antibody; CSBP antibody; CSBP1 antibody; CSBP2 antibody; CSPB1 antibody; Cytokine suppressive anti-inflammatory drug-binding protein antibody; EXIP antibody; MAP kinase 14 antibody; MAP kinase MXI2 antibody; MAP kinase p38 alpha antibody; MAPK 14 antibody; MAPK14 antibody; MAX interacting protein 2 antibody; MAX-interacting protein 2 antibody; Mitogen Activated Protein Kinase 14 antibody; Mitogen activated protein kinase p38 alpha antibody; Mitogen-activated protein kinase 14 antibody; Mitogen-activated protein kinase p38 alpha antibody; MK14_HUMAN antibody; Mxi 2 antibody; MXI2 antibody; p38 ALPHA antibody; p38 antibody; p38 MAP kinase antibody; p38 MAPK antibody; p38 mitogen activated protein kinase antibody; p38ALPHA antibody; p38alpha Exip antibody; PRKM14 antibody; PRKM15 antibody; RK antibody; SAPK2A antibody; Stress-activated protein kinase 2a antibody
Immunogen Description	Peptide sequence around phosphorylation site of tyrosine 182 (T-G-Y(p)-V-A) derived from Human P38 MAPK.
Immunogen Species	Human
Immunogen Sequence	Complete sequences for the immunogen, target protein, and peptides are available upon request.
Clonality	Polyclonal
Purification Method	Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
Buffer	Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form	Liquid
Application	ELISA, WB, IHC
Storage Conditions	Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.