Rabbit anti-Human ATP6V1G1 Polyclonal Antibody

Name: Rabbit anti-Human ATP6V1G1 Polyclonal Antibody
Brand: Beta LifeScience
SKU: BLC-06273A
Price: 299.0 USD
Availability: InStock

Immunohistochemistry of paraffin-embedded human colon cancer using ATP6V1G1 Antibody at dilution of 1:50

Immunofluorescence staining of PC-3 cells with ATP6V1G1 Antibody at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Product Overview

Product Title	Rabbit anti-Human ATP6V1G1 Polyclonal Antibody
Description	The antibody against ATP6V1G1 was raised in rabbit using the Recombinant Human V-type proton ATPase subunit G 1 protein (2-118AA) as the immunogen. This antibody exists as a non-conjugated isotype IgG, purified by protein G with a purity greater than 95%. This antibody has been validated on ELISA, WB, IHC, IF.
Uniprot Id - Alink	O75348
Host Species	Rabbit
Reactivity	Human
Target Name	ATP6V1G1
Target Synonyms	ATP 6G antibody; ATP 6J antibody; ATP6G antibody; ATP6G1 antibody; ATP6GL antibody; Atp6v1g1 antibody; ATPase H+ transporting lysosomal (vacuolar proton pump) member J antibody; ATPase H+ transporting lysosomal 13kD V1 subunit G isoform 1 antibody; ATPase H+ transporting lysosomal 13kDa V1 subunit G1 antibody; ATPase H+ transporting lysosomal member J antibody; ATPase H+ transporting lysosomal subunit G isoform 1 antibody; DKFZp547P234 antibody; V ATPase 13 kDa subunit 1 antibody; V ATPase G subunit 1 antibody; V-ATPase 13 kDa subunit 1 antibody; V-ATPase subunit G 1 antibody; V-type proton ATPase subunit G 1 antibody; Vacuolar ATP synthase subunit G 1 antibody; Vacuolar ATP synthase subunit G1 antibody; Vacuolar ATP synthase subunit M16 antibody; Vacuolar H+ ATPase G1 antibody; Vacuolar H+ ATPase subunit G 1 antibody; Vacuolar proton pump G subunit 1 antibody; Vacuolar proton pump subunit G 1 antibody; Vacuolar proton pump subunit M16 antibody; VATG1_HUMAN antibody; Vma 10 antibody; Vma10 antibody
Immunogen Description	Recombinant Human V-type proton ATPase subunit G 1 protein (2-118AA)
Immunogen Species	Human
Immunogen Sequence	Complete sequences for the immunogen, target protein, and peptides are available upon request.
Conjugate	Non-conjugated
Clonality	Polyclonal
Isotype	IgG
Purification Method	>95%, Protein G purified
Buffer	0.03% Proclin 300Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Form	Liquid
Application	ELISA, WB, IHC, IF
Storage Conditions	Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.