Rabbit anti-Human ATP6V1B1 Polyclonal Antibody

Name: Rabbit anti-Human ATP6V1B1 Polyclonal Antibody
Brand: Beta LifeScience
SKU: BLC-05848A
Price: 299.0 USD
Availability: InStock

Immunohistochemistry of paraffin-embedded human salivary gland tissue using ATP6V1B1 Antibody at dilution of 1:100

Immunohistochemistry of paraffin-embedded human kidney tissue using ATP6V1B1 Antibody at dilution of 1:100

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Product Overview

Product Title	Rabbit anti-Human ATP6V1B1 Polyclonal Antibody
Description	The antibody against ATP6V1B1 was raised in rabbit using the Recombinant Human V-type proton ATPase subunit B, kidney isoform protein (1-280AA) as the immunogen. This antibody exists as a non-conjugated isotype IgG, Antigen affinity purified. This antibody has been validated on ELISA, WB, IHC.
Uniprot Id - Alink	P15313
Host Species	Rabbit
Reactivity	Human, Mouse
Target Name	ATP6V1B1
Target Synonyms	Atp6b1 antibody; ATP6V1B1 antibody; ATPase; H+ transporting; lysosomal (vacuolar proton pump); beta 56/58 kDa; isoform 1 antibody; ATPase; H+ transporting; lysosomal 56/58kDa; V1 subunit B; isoform 1 antibody; ATPase; H+ transporting; V1 subunit B; isoform 1 antibody; Endomembrane proton pump 58 kDa subunit antibody; H(+) transporting two sector ATPase 58kD subunit antibody; H+ ATPase beta 1 subunit antibody; kidney isoform antibody; Lysosomal 56/58kDa antibody; MGC32642 antibody; RTA1B antibody; V ATPase B1 subunit antibody; V type proton ATPase subunit B kidney isoform antibody; V type proton ATPase subunit B; kidney isoform antibody; V-ATPase subunit B 1 antibody; V-type proton ATPase subunit B antibody; Vacuolar proton pump 3 antibody; Vacuolar proton pump subunit 3 antibody; Vacuolar proton pump subunit B 1 antibody; VATB antibody; VATB1_HUMAN antibody; VMA2 antibody; Vpp 3 antibody; Vpp3 antibody
Immunogen Description	Recombinant Human V-type proton ATPase subunit B, kidney isoform protein (1-280AA)
Immunogen Species	Human
Immunogen Sequence	Complete sequences for the immunogen, target protein, and peptides are available upon request.
Conjugate	Non-conjugated
Clonality	Polyclonal
Isotype	IgG
Purification Method	Antigen affinity purified
Buffer	PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Form	Liquid
Application	ELISA, WB, IHC
Storage Conditions	Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.