Rabbit anti-Human ATP2B4 Polyclonal Antibody

Name: Rabbit anti-Human ATP2B4 Polyclonal Antibody
Brand: Beta LifeScience
SKU: BLC-00123A
Availability: InStock

IHC image of the antibody diluted at 1:100 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

IHC image of ATP2B4 Antibody diluted at 1:100 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunofluorescence staining of Hela cells with ATP2B4 Antibody at 1:33, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Product Overview

Product Title	Rabbit anti-Human ATP2B4 Polyclonal Antibody
Description	The antibody against ATP2B4 was raised in rabbit using the Recombinant Human Plasma membrane calcium-transporting ATPase 4 protein (281-345AA) as the immunogen. This antibody exists as a non-conjugated isotype IgG, purified by protein G with a purity greater than 95%. This antibody has been validated on ELISA, IHC, IF.
Uniprot Id - Alink	P23634
Host Species	Rabbit
Reactivity	Human
Target Name	ATP2B4
Target Synonyms	AT2B4_HUMAN antibody; ATP2B2 antibody; Atp2b4 antibody; ATPase Ca++ transporting plasma membrane 4 antibody; Cation transporting ATPase antibody; DKFZp686G08106 antibody; DKFZp686M088 antibody; Matrix remodelling associated 1 antibody; Matrix remodelling associated protein 1 antibody; Matrix-remodeling-associated protein 1 antibody; MXRA 1 antibody; MXRA1 antibody; OTTHUMP00000034088 antibody; OTTHUMP00000034089 antibody; Plasma membrane calcium ATPase 4 antibody; Plasma membrane calcium ATPase isoform 4 antibody; Plasma membrane calcium pump isoform 4 antibody; Plasma membrane calcium transporting ATPase 4 antibody; Plasma membrane calcium-transporting ATPase 4 antibody; PMCA 4 antibody; PMCA4 antibody; PMCA4b antibody; PMCA4x antibody; Sarcolemmal calcium pump antibody
Immunogen Description	Recombinant Human Plasma membrane calcium-transporting ATPase 4 protein (281-345AA)
Immunogen Species	Human
Immunogen Sequence	Complete sequences for the immunogen, target protein, and peptides are available upon request.
Conjugate	Non-conjugated
Clonality	Polyclonal
Isotype	IgG
Purification Method	>95%, Protein G purified
Buffer	0.03% Proclin 300Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form	Liquid
Application	ELISA, IHC, IF
Storage Conditions	Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.