Rabbit anti-Human ATP2A1 Polyclonal Antibody

Name: Rabbit anti-Human ATP2A1 Polyclonal Antibody
Brand: Beta LifeScience
SKU: BLC-03214A
Price: 299.0 USD
Availability: InStock

The image on the left is immunohistochemistry of paraffin-embedded Human prostate cancer tissue using the antibody Antibody) at dilution 1/35, on the right is treated with synthetic peptide. (Original magnification: ×200)

The image on the left is immunohistochemistry of paraffin-embedded Human brain tissue using ATP2A1 Antibody(ATP2A1 Antibody) at dilution 1/35, on the right is treated with synthetic peptide. (Original magnification: ×200)

Gel: 6%SDS-PAGE, Lysate: 40 μg, Lane 1-2: NIH/3T3 cells, LoVo cells, Primary antibody: ATP2A1 Antibody(ATP2A1 Antibody) at dilution 1/500, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 3 minutes

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Product Overview

Product Title	Rabbit anti-Human ATP2A1 Polyclonal Antibody
Description	The antibody against ATP2A1 was raised in rabbit using the Synthetic peptide of Human ATP2A1 as the immunogen. This antibody exists as a non-conjugated isotype IgG, Antigen affinity purified. This antibody has been validated on ELISA, WB, IHC.
Uniprot Id - Alink	O14983
Host Species	Rabbit
Reactivity	Human, Mouse
Target Name	ATP2A1
Target Synonyms	fast twitch skeletal muscle isoform antibody; AT2A1_HUMAN antibody; ATP2A antibody; ATP2A1 antibody; ATPase Ca++ transporting cardiac muscle fast twitch 1 antibody; ATPase Ca++ transporting fast twitch 1 antibody; ATPase; Ca(2+)-transporting fast twitch 1 antibody; Calcium pump 1 antibody; Calcium transporting ATPase sarcoplasmic reticulum type fast twitch skeletal muscle isoform antibody; Calcium-transporting ATPase sarcoplasmic reticulum type antibody; EC 3.6.3.8 antibody; Endoplasmic reticulum class 1/2 Ca(2+) ATPase antibody; Fast skeletal muscle SR calcium ATPase antibody; OTTHUMP00000162561 antibody; OTTHUMP00000162562 antibody; Sarcoendoplasmic reticulum calcium ATPase antibody; Sarcoplasmic reticulum Ca(2+)-ATPase 1 antibody; Sarcoplasmic/endoplasmic reticulum calcium ATPase 1 antibody; SERCA 1 antibody; SERCA1 antibody; SERCA1 truncated isoform; included antibody; SR Ca(2+) ATPase 1 antibody; SR Ca(2+)-ATPase 1 antibody
Immunogen Description	Synthetic peptide of Human ATP2A1
Immunogen Species	Human
Immunogen Sequence	Complete sequences for the immunogen, target protein, and peptides are available upon request.
Conjugate	Non-conjugated
Clonality	Polyclonal
Isotype	IgG
Purification Method	Antigen affinity purified
Buffer	pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Form	Liquid
Application	ELISA, WB, IHC
Storage Conditions	Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.