Rabbit anti-Human ARHGAP18 Polyclonal Antibody

Name: Rabbit anti-Human ARHGAP18 Polyclonal Antibody
Brand: Beta LifeScience
SKU: BLC-00741A
Price: 299.0 USD
Availability: InStock

Western Blot | Positive WB detected in: Hela whole cell lysate, NIH/3T3 whole cell lysate, A549 whole cell lysate, Rat lung tissue, Rat kidney tissue, Mouse heart tissue | All lanes: ARHGAP18 antibody at 4.5µg/ml | Secondary | Goat polyclonal to rabbit IgG at 1/50000 dilution | Predicted band size: 75, 71 kDa | Observed band size: 75 kDa

IHC image of ARHGAP18 Antibody diluted at 1:200 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunofluorescence staining of HepG2 cells with ARHGAP18 Antibody at 1:66, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Product Overview

Product Title	Rabbit anti-Human ARHGAP18 Polyclonal Antibody
Description	The antibody against ARHGAP18 was raised in rabbit using the Recombinant Human Rho GTPase-activating protein 18 protein (389-577AA) as the immunogen. This antibody exists as a non-conjugated isotype IgG, purified by protein G with a purity greater than 95%. This antibody has been validated on ELISA, WB, IHC, IF.
Uniprot Id - Alink	Q8N392
Host Species	Rabbit
Reactivity	Human, Rat, Mouse
Target Name	ARHGAP18
Target Synonyms	4833419J07Rik antibody; AI314906 antibody; AI843501 antibody; Arhgap18 antibody; bA307O14.2 antibody; FLJ25728 antibody; MacGAP antibody; MGC126757 antibody; MGC138145 antibody; OTTHUMP00000017190 antibody; OTTMUSP00000028606 antibody; RHG18_HUMAN antibody; Rho GTPase-activating protein 18 antibody; Rho-type GTPase-activating protein 18 antibody; SENEX antibody
Immunogen Description	Recombinant Human Rho GTPase-activating protein 18 protein (389-577AA)
Immunogen Species	Human
Immunogen Sequence	Complete sequences for the immunogen, target protein, and peptides are available upon request.
Conjugate	Non-conjugated
Clonality	Polyclonal
Isotype	IgG
Purification Method	>95%, Protein G purified
Buffer	0.03% Proclin 300Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form	Liquid
Application	ELISA, WB, IHC, IF
Storage Conditions	Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.