Recombinant Cynomolgus CD3E&CD3G/CD3 epsilon&CD3 gamma Protein, Active

Name: Recombinant Cynomolgus CD3E&CD3G/CD3 epsilon&CD3 gamma Protein, Active
Brand: Beta LifeScience
SKU: BLK-00382P-100UG
Availability: InStock

Cynomolgus CD3E&CD3G on Tris-Bis PAGE under reduced condition. The purity is greater than 90%.

Collections: High-quality recombinant proteins

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Product Overview

Description	Recombinant Cynomolgus CD3E&CD3G/CD3 epsilon&CD3 gamma Protein is expressed from HEK293 with His tag and Flag tag at the C-Terminus.It contains Gln22-Asp117(CD3E) & Gln23-Thr113(CD3G).
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Accession	Q95LI5.2(CD3E)&Q95LI7(CD3G)
Target Symbol	CD3E&CD3G/CD3 epsilon&CD3 gamma
Species	Cynomolgus
Expression System	HEK293
Tag	C-His-Flag
Expression Range	Gln22-Asp117(CD3E) & Gln23-Thr113(CD3G)
Mol. Weight	The protein has a predicted MW of 11.93 kDa (CD3E) and 11.52 kDa (CD3G). Due to glycosylation, the protein migrates to 13-23 kDa based on Tris-Bis PAGE result.
Form	Liquid
Formulation	Supplied as 0.22um filtered solution in PBS (pH 7.4).
Endotoxin	Less than 1EU per ug by the LAL method.
Activity	Immobilized Cynomolgus CD3E&CD3G, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-CD3 Antibody, hFc Tag with the EC50 of 5.1ng/ml determined by ELISA. Contact us for detailed testing images.
Storage	Valid for 12 months from date of receipt when stored at -80°C.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Shipping	Shipped with dry ice.
Gene Background	T-cell surface glycoprotein CD3 epsilon & CD3 gamma chain, also known as CD3E & CD3G , are single-pass type I membrane proteins.When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain.

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.