Biotinylated Recombinant Mouse Cholesterol Side-Chain Cleavage Enzyme, Mitochondrial (CYP11A1) Protein (MBP&His-Avi)

Name: Biotinylated Recombinant Mouse Cholesterol Side-Chain Cleavage Enzyme, Mitochondrial (CYP11A1) Protein (MBP&His-Avi)
Brand: Beta LifeScience
SKU: BLC-00232P
Price: 815.2 USD
Availability: InStock

Greater than 85% as determined by SDS-PAGE.

Collections: Avi-tagged proteins, Biotinylated proteins, Featured biotinylated protein molecules, Other recombinant proteins, Recombinant proteins

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Product Overview

Description	Biotinylated Recombinant Mouse Cholesterol Side-Chain Cleavage Enzyme, Mitochondrial (CYP11A1) Protein (MBP&His-Avi) is produced by our E.coli expression system. This is a full length protein.
Purity	Greater than 85% as determined by SDS-PAGE.
Activity	Not tested.
Uniprotkb	Q9QZ82
Target Symbol	CYP11A1
Synonyms	(CYPXIA1)(Cholesterol desmolase)(Cytochrome P450 11A1)(Cytochrome P450(scc)
Species	Mus musculus (Mouse)
Expression System	E.coli
Tag	N-MBP&C-6His-Avi
Target Protein Sequence	TSTSSPRSFNEIPSPGDNGWLNLYHFWRESGTQKIHYHQMQSFQKYGPIYREKLGTLESVYIVDPKDASILFSCEGPNPERFLVPPWVAYHQYYQRPIGVLFKSSDAWKKDRIVLNQEVMAPGAIKNFVPLLEGVAQDFIKVLHRRIKQQNSGNFSGVISDDLFRFSFESISSVIFGERMGMLEEIVDPEAQRFINAVYQMFHTSVPMLNLPPDFFRLLRTKTWKDHAAAWDVIFNKADEYTQNFYWDLRQKRDFSQYPGVLYSLLGGNKLPFKNIQANITEMLAGGVDTTSMTLQWNLYEMAHNLKVQEMLRAEVLAARRQAQGDMAKMVQLVPLLKASIKETLRLHPISVTLQRYTVNDLVLRNYKIPAKTLVQVASFAMGRDPGFFPNPNKFDPTRWLEKSQNTTHFRYLGFGWGVRQCLGRRIAELEMTILLINLLENFRIEVQNLRDVGTKFSLILMPENPILFNFQPLKQDLGPAVTRKDNTVN
Expression Range	37-526aa
Protein Length	Full Length of Mature Protein
Mol. Weight	104.4 kDa
Research Area	Cancer
Form	Liquid or Lyophilized powder
Buffer	Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution	Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	A cytochrome P450 monooxygenase that catalyzes the side-chain hydroxylation and cleavage of cholesterol to pregnenolone, the precursor of most steroid hormones. Catalyzes three sequential oxidation reactions of cholesterol, namely the hydroxylation at C22 followed with the hydroxylation at C20 to yield 20R,22R-hydroxycholesterol that is further cleaved between C20 and C22 to yield the C21-steroid pregnenolone and 4-methylpentanal. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate and reducing the second into a water molecule. Two electrons are provided by NADPH via a two-protein mitochondrial transfer system comprising flavoprotein FDXR (adrenodoxin/ferredoxin reductase) and nonheme iron-sulfur protein FDX1 or FDX2 (adrenodoxin/ferredoxin).
Subcellular Location	Mitochondrion inner membrane; Peripheral membrane protein.
Protein Families	Cytochrome P450 family
Database References	KEGG: mmu:13070 STRING: 10090.ENSMUSP00000034874 UniGene: Mm.302865

Gene Functions References

melatonin not only elevated progesterone (P) secretion, but also upregulated expressions of StAR and Cyp11a1 and also had an increased Ihh expression in endometrium. PMID: 28749439
reduction in expression mirrored reduction in adrenal corticosterone output PMID: 29046340
The results demonstrated that ZnT7 gene silencing downregulated the expression of StAR, P450scc and 3beta-HSD as well as progesterone concentrations in the human chorionic gonadotrophin-stimulated Leydig tumor cells. PMID: 27121848
1,25D3 modulates CD8+ T cell phenotype via recruitment of the VDR transcription factor to the promoter region of Cyp11a1 leading to prevention of lung allergic responses. PMID: 26750596
Data from Cyp11a1 transgenic mice suggest that overexpression of Cyp11a1 results in reduced pregnancy rate, impaired implantation/placentation, decreased litter size, and stillbirth; overexpression of Cyp11a1 disrupts development of corpus luteum. PMID: 23966322
Cyp11a1 plays an important role in the development of peanut allergy, regulating peanut-induced allergic responses through effects on steroidogenesis, an essential pathway in T(H)2 differentiation. PMID: 23870673
Cyp11a1 controls the phenotypic conversion of CD8(+) T cells from IFN-gamma to IL-13 production, linking steroidogenesis in CD8(+) T cells, a nonclassical steroidogenic tissue, to a proallergic differentiation pathway PMID: 23630275
Sodium fluoride inhibited expression of StAR and P450scc in mouse Leydig tumor cells, which inhibited progesterone secretion. PMID: 22443068
LH/hCG tightly regulates MKP-2 expression, which modulates the induction of CYP11A1 by 8Br-cAMP. PMID: 23471219
Cyp11a1 null embryos had adrenal medulla with abnormal mitochondria. Cells did not synthesized no epinephrine. Decreased diencephalon Hsd11b1, increased Crh, and increased pituitary Pomc expression, thus higher plasma ACTH PMID: 22962254
Data suggest that the 5' flanking region of CYP11A gene contains sequences between 2.5 and 5 kb that are necessary for expression of mouse CYP11A in Leydig cells but not in adrenal cells. [REVIEW] PMID: 22217834
Daxx, a HIPK kinase substrate in the apoptosis pathway, was phosphorylated by HIPK3 at Ser-669 in response to cAMP stimulation. PMID: 22199361
Prenatal exposure to ethanol increased expressions of StAR and P450scc in maternal adrenal and decreased them in fetal adrenal. PMID: 20627497
Pubertal Cd exposure markedly reduced mRNA and protein levels of testicular StAR, P450scc, P450(17alpha) and 17beta-HSD in mice. PMID: 19897027
Transcription of cholesterol side-chain cleavage cytochrome P450 in the placenta: activating protein-2 assumes the role of steroidogenic factor-1 by binding to an overlapping promoter element. PMID: 12145340
Cyp11a1 null mice display a lack of steroid production, abnormal gene expression, and aberrant reproductive organ development resemble various steroid deficiency syndromes. PMID: 12145347
Male CYP11A1 null mice are feminized and their adrenals and genitalia accumulate excessive amounts of lipid PMID: 12530664
Proximal SF-1 binding site plays important role in regulating tissue-specific expression of Cyp11a1 and stress response. PMID: 15666831
This study sought to elucidate overlooked modes of regulation of P450scc transcription in the rodent placenta and ovary. PMID: 17213386
SF-1 and AP-1 cooperate to activate CYP11A1 transcription in vitro and in vivo. PMID: 17218410
Data show that HDAC inhibitors suppressed the expression of steroidogenic gene CYP11A1 and decreased steroid secretion by increasing the ubiquitination and degradation of SF-1, a factor important for the transcription of all steroidogenic genes. PMID: 17709382
Data show that the mutation of the SF-1 response element of Cyp11a1 results in reduced stress response due to decreased adrenal CYP11A1 expression and insufficient stress-induced glucocorticoids secretion. PMID: 18174359
Heterologous expression in yeast of mCYP11A1 fusions with different topogenic signals of yeast mitochondrial proteins was used to gain insight in the mechanism of its topogenesis in mitochondria. PMID: 18621100
Lycopene protects against PCB-induced changes in Leydig cellular StAR protein, cytochrome P450 scc expression and activity of 3beta-HSD. PMID: 19071209

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.