Biotinylated Human Carbonic Anhydrase 9 Protein (C-Avi-6His)

Beta LifeScience SKU/CAT #: BL-2399NP
BL-2399NP: Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
BL-2399NP: Greater than 95% as determined by reducing SDS-PAGE. (QC verified)

Biotinylated Human Carbonic Anhydrase 9 Protein (C-Avi-6His)

Beta LifeScience SKU/CAT #: BL-2399NP
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Product Overview

Description Biotinylated Recombinant Human Carbonic Anhydrase 9 is produced by our Mammalian expression system and the target gene encoding Gln38-Asp414 is expressed with a 6His, Avi tag at the C-terminus.
Accession Q16790
Synonym CA9; CA-IX; Carbonic Anhydrase IX; Carbonate dehydratase IX; G250; MN; P54/58N; RCC; RCC-associated protein G250
Gene Background Carbonic anhydrases IX (CA IX), also known as membrane antigen MN or CA9, is a member of the carbonic anhydrase (CA) family and may be involved in cell proliferation and cellular transformation. CAs are zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide (H2O + CO2 = H+ + HCO3–) and thus participate in a variety of biological and physical processes. CA9 is a transmembrane enzyme expressed primarily in carcinoma cells. It is one of the best markers for hypoxia and for RCC. Appears to be a novel specific biomarker for a cervical neoplasia.
Molecular Mass 43.6 KDa
Apmol Mass 60 KDa, reducing conditions
Formulation Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
Endotoxin Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test.
Purity Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
Biological Activity Not tested
Reconstitution Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Storage Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below.
Usage For Research Use Only

Target Details

Target Function Reversible hydration of carbon dioxide. Participates in pH regulation. May be involved in the control of cell proliferation and transformation. Appears to be a novel specific biomarker for a cervical neoplasia.
Subcellular Location Nucleus. Nucleus, nucleolus. Cell membrane; Single-pass type I membrane protein. Cell projection, microvillus membrane; Single-pass type I membrane protein. Note=Found on the surface microvilli and in the nucleus, particularly in nucleolus.
Protein Families Alpha-carbonic anhydrase family
Database References
Tissue Specificity Expressed primarily in carcinoma cells lines. Expression is restricted to very few normal tissues and the most abundant expression is found in the epithelial cells of gastric mucosa.

Gene Functions References

  1. Our findings indicated that CA-IX may be a potential prognostic biomarker in the treatment of glioblastoma multiforme PMID: 29952031
  2. Our results do not suggest a prognostic role for CA IX overexpression in stage III non-small cell lung cancer patients who received neoadjuvant treatment. PMID: 30029935
  3. FZD1 and CAIX might be important biological markers for the carcinogenesis, metastasis, invasion, and prognosis of pancreatic ductal adenocarcinoma PMID: 28921449
  4. High expression of CA9 is associated with breast cancer. PMID: 29893327
  5. Data suggest that the conformational flexibility may have several important roles in tumour progression, facilitating interactions of carbonic anhydrase IX (hCA IX) with partner proteins assisting tumour spreading and progression. PMID: 29564477
  6. the findings of this study establish the SOX9/CA9-mediated oncogenic pathway in glioma, the inhibition of which enhances the sensitivity of glioma cells to Temozolomide (TMZ) treatment, and thus highlights the value of developing small molecules or antibodies against the SOX9/CA9 pathway, for combination therapy with TMZ, in the more efficient management of glioma PMID: 29749469
  7. CA9 SNP rs1048638 and haplotype A1AA are associated with the susceptibility of cervical invasive squamous cell carcinoma for Taiwanese women PMID: 29725249
  8. The TCGA data revealed that CAIX expression was significantly higher in bladder cancer specimens than in normal tissue. PMID: 29949785
  9. CAIX enzyme inhibition assay showed the IC50 values in nM range. Though all the three compounds (1-3) showed a good binding with CAIX, compound 2 showed the best inhibition of CAIX activity. These compounds were non-toxic on normal cell lines (HEK-293) and significantly inhibit the proliferation of hypoxic cancer cells PMID: 28830777
  10. CA9 expression was observed in normal islets, while neuroendocrine microadenomas and small (< 1 cm) pancreatic neuroendocrine neoplasms/tumors showed CA9 expression loss. PMID: 29666945
  11. Study revealed that CA9 expression was a pivotal predictive factor for poor prognosis after radical surgery for HCC. Moreover, the CA9 regulation of the expression of EMT-related molecules represented a mechanism that enhanced malignant potential. PMID: 28849188
  12. Report weak CA9 immunoreactivity in majority of CA9 positive colorectal carcinoma cases associated with macroscopic growth pattern. PMID: 28554753
  13. Molecular characterization of carbonic anhydrase IX catalytic domain has been presented. PMID: 27373313
  14. Authors present evidence that enhanced autophagy does not play a role in the enhanced growth of the CA9+ cells. Our study suggests a direct in vivo functional link between hypoxic cells and CICs in primary cervix cancer xenografts. PMID: 27901496
  15. Genetic disruption of the intracellular pH-regulating proteins Na+/H+ exchanger 1 (SLC9A1) and carbonic anhydrase 9 reduces the proliferation of colon cancer cells. PMID: 28055960
  16. Data show that targeting the hypoxic tumor compartment by knockdown of carbonic anhydrase IX (CAIX) using short hairpin RNA or by chemical inhibition of CAIX with acetazolamide potentiates the anti-cancer activity of mTOR inhibitors rapamycin. PMID: 27153561
  17. Authors demonstrate that CAIX associates with MMP14 through potential phosphorylation residues within its intracellular domain, and that CAIX enhances MMP14-mediated collagen degradation by directly contributing hydrogen ions required for MMP14 catalytic activity. PMID: 28692057
  18. Results provide evidence that CAIX induces chemoresistance of A549 cells, a lung cancer cell line. PMID: 28028936
  19. The results suggest the possibility that CA9 exosomes released from hypoxic RCC may enhance angiogenesis in microenvironment, thereby contributing to cancer progression. PMID: 28851650
  20. CA9 expression is highly associated with distant metastases, including para-aortic involvement. PMID: 27102843
  21. Increased miR-210 and concomitant decreased ISCU RNA levels were found in ~40% of tumors and this was significantly associated with HIF-1alpha and CAIX, but not MCT1 or MCT4, over-expression. PMID: 28099149
  22. describe the identification of the structural determinants responsible for the CA IX/CAND1 interaction PMID: 28388044
  23. Data suggest that CAIX (carbonic anhydrase IX) is a novel downstream mediator of asTF (alternatively spliced tissue factor) in pancreatic ductal adenocarcinoma, particularly under hypoxic conditions that model late-stage tumor microenvironment; tumor hypoxia appears to lead to up-regulation of CAIX expression (or 'activation'), which is more pronounced in tumor cells overexpressing asTF. PMID: 27721473
  24. Our findings suggest that LCN2 suppresses tumour metastasis by targeting the transcriptional and post-transcriptional regulation of CAIX in oral squamous cell carcinoma PMID: 27207653
  25. The findings suggest that CA9, and particularly its carbonic anhydrase activity, promotes the tumorigenicity of adult T-cell leukemia/lymphoma-derived cells and may be involved in malignant development of lymphoma-type adult T-cell leukemia/lymphoma. PMID: 28075522
  26. We demonstrated that the expression levels of glycolysis-related proteins glucose transporter 1, hexokinase II, carbonic anhydrase IX, and monocarbonylate transporter 4 differ between thyroid cancer subtypes and are correlated with poorer prognosis PMID: 28347233
  27. CAIX and PTEN had prognostic importance for metastatic renal cell carcinoma patients receiving first-line VEGFR TKI. Future validation and mechanistic studies are required. PMID: 26526582
  28. combined HIF1alpha and CAIX protein expression may serve as an unfavorable prognostic indicator particularly in patients treated with cyclophosphamide-based chemotherapy or radiotherapy as well as those with basal phenotype of breast cancer PMID: 27184798
  29. CA9 may have a role in castration-resistant prostate cancer progression PMID: 27630286
  30. The diagnostic accuracy of CA9 expression for clear cell renal cell carcinoma was 100%. PMID: 27775441
  31. High expression of CA IX was associated with pancreatic cancer. PMID: 26224207
  32. CAIX mRNA expression was significantly higher (p < 0.05) in hypoxia for all cell lines, which was in agreement with protein expression by ICC. CAXII expression was mixed, with a modest hypoxia-related increase in two cell lines (p < 0.05) and no change in others. PMID: 26276155
  33. We also demonstrated that the cell-surface CA IX level dropped during the death progress due to an increased ECD shedding, which required a functional ADAM17. Inhibitors of metalloproteinases reduced CA IX ECD shedding, but not apoptosis. PMID: 26993100
  34. In neuroblastoma cells, CAIX and PGK1 expression is up regulated under hypoxia and correlates with response to targeted anti-proliferative treatment. PMID: 26510737
  35. Flow cytometrically sorted CA9+ population showed increased mRNA level of a Wnt signaling factor AXIN2. In conclusion, these observations indicate that CA9 expression in normal crypt base cells has association with intestinal epithelial stemness PMID: 26648507
  36. We used cobalt chloride (CoCl2) as a hypoxia-mimetic agent and found that the expression of HIF-1a protein, CA IX mRNA and protein, is effectively upregulated, except for HIF-1a mRNA. PMID: 26648580
  37. We conclude that CA9/miR34 interplay shares in the hypoxic regulation of mammospheres and therefore, may play a relevant role in the hypoxic breast cancer stem cell niche. PMID: 26553365
  38. Knockdown of CAIX significantly reduced proliferation of cancer cells, suggesting that rapid efflux of lactate and H(+), as enhanced by CAIX, contributes to cancer cell survival under hypoxic conditions. PMID: 26337752
  39. Data indicate that carbonic anhydrase IX (CAIX) inhibition as a relevant therapeutic goal in breast cancer, targeting the migratory, invasive, and metastatic potential of this disease. PMID: 26259239
  40. Inhibition of CA9 expression or activity resulted in radiation sensitization of RCC in a preclinical mouse model. PMID: 26252502
  41. Urinary CAIX has a high sensitivity and specificity for diagnosing urothelial bladder cancer. PMID: 26138037
  42. The intrinsic thermodynamic parameters of compound binding to CA IX helped to draw the compound structure to thermodynamics relationship. PMID: 26794023
  43. our study showed that the expression of CAIX in oral squamous cell carcinoma (OSCC) samples can predict the progression of OSCC and survival of OSCC patients in Taiwan PMID: 26130414
  44. we aimed to determine the effect of immunohistochemical staining of ezrin, carbonic anhydrase IX (CA IX), and neuropilin-2 on the prognosis of patients diagnosed with metastatic RCC who were treated with TKIs between January 2007 and June 2012. PMID: 26026587
  45. CA IX is expressed in B-cell lymphomas and is qualitatively correlated with extracellular acidosis in xenograft tumor models. PMID: 25130478
  46. We have developed an efficient system for the production of the catalytic domain of CA IX in methylotrophic yeast Pichia pastoris PMID: 26522624
  47. CAIX expression is increased in hypoxia to compensate for the decrease in its activity produced by a low extracellular pH. A major function of CAIX is to lower the extracellular pH. PMID: 26249175
  48. A strong positive correlation between the mRNA expression levels of HIF-2alpha, CA9, VEGF, GLUT-1 and OPN suggests a specific hypoxia-associated profile of mRNA expression in glioblastoma multiforme PMID: 25963717
  49. expression of the CA-IX protein is a crucial predictor of poor prognosis in resectable hepatocellular carcinoma PMID: 25738958
  50. Preventing carbonic anhydrase IX association with 45 S rDNA gene promoters. PMID: 25793203


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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.

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