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Recombinant Interleukin-4 (IL-4) Proteins
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Recombinant human IL-4 is a key tool in modern immunology and inflammation-related research. Known for its broad influence on immune responses, recombinant IL-4 plays an essential role in studies focused on allergy, autoimmune diseases, asthma, and tissue repair. At Beta LifeScience, we offer highly purified and biologically active interleukin 4 recombinant human IL-4 proteins produced through advanced expression systems to ensure consistency and reproducibility in every experiment.
Whether you’re developing biologics, studying cytokine signaling pathways, or screening drug candidates, our human recombinant IL-4 proteins provide the precision and performance trusted by scientists worldwide.
The Role of IL-4 in the Immune System
Interleukin-4 is a multifunctional cytokine that acts as a key mediator of immune regulation. It is primarily secreted by activated T helper 2 (Th2) cells, mast cells, eosinophils, and basophils. IL-4 is crucial in promoting the differentiation of naïve T cells into Th2 cells while suppressing Th1 differentiation. This cytokine is also known to support B cell proliferation and enhance immunoglobulin class switching to IgE and IgG1, which are central to allergic reactions and antibody-mediated immunity.
Because of its central role in immune signaling, interleukin 4 is often implicated in chronic inflammatory conditions, including asthma, dermatitis, and other hypersensitivity disorders. Recombinant human IL-4 allows researchers to explore these pathways in greater depth with controlled dosing and reliable purity.
Why Use Recombinant IL-4?
Using recombinant IL-4 instead of endogenous sources provides unmatched control and reproducibility in experimental settings. Human recombinant IL-4 proteins are engineered to mirror the natural molecule while eliminating contaminants and variability associated with cell extracts or serum.
Key benefits include:
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High purity for sensitive applications
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Lot-to-lot consistency to reduce experimental variability
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Defined biological activity validated through standardized assays
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Compatibility with cell-based, in vivo, and biochemical assays
Researchers can confidently explore IL-4’s effects in a wide range of immunological and inflammatory models using Beta LifeScience’s recombinant human IL-4 products.
Resources
IL-4 Blog Articles
The Crucial Role of Cytokines in Biomedical Research and Drug Development
Species Variants Available
While recombinant human IL-4 is the most in-demand form, Beta LifeScience also provides interleukin 4 proteins from other species to support comparative studies and animal models. These include:
Featured Products
| Cat. No. | Species | Product Description | Host | Structure | Purity | Activity | Features |
|---|---|---|---|---|---|---|---|
| BLC-05423P | Human | Human IL-4 Protein, Active | Mammalian cell | P05112 (25-153aa) | ≥ 95% | ED50: < 0.2 ng/mL | SDS-PAGE, Cell Based Assay |
| BLC-05428P | Human | Human IL-4 Protein (His), Active | Mammalian cell | P05112 (25-153aa) | ≥ 95% | ED50 < 0.2 ng/mL | SDS-PAGE, Cell Based Assay |
| BL-2119NP | Human | Human IL-4 Protein, Active | Mammalian cell | P05112 (25-153aa) | ≥ 95% | ED50: 0.01-0.05 ng/mL | SDS-PAGE, Cell Based Assay, BLI |
| Human | Human IL-4 Protein, Active | Mammalian cell | P05112 (25-153aa) | ≥ 95% | ED50 ≤ 0.2 ng/mL | SDS-PAGE, Cell Based Assay | |
| BL-2129NP | Human | Human IL-4 Protein (C-6His), Active | Mammalian cell | P05112 (25-153aa) | ≥ 95% | ED50: 0.05-0.5 ng/mL | SDS-PAGE, HPLC, Cell Based Assay, BLI |
| BL-2092NP | Human | Human IL-4 Protein (C-Fc), Active | Mammalian cell | P05112 (25-153aa) | ≥ 95% | BLI: affinity constant of <10-3 nM | SDS-PAGE, HPLC, ELISA, BLI |
| BLC-05400P | Human | Human IL-4 Protein, Active | E.coli | P05112 (25-153aa) | ≥ 95% | ED50: < 2 ng/mL | SDS-PAGE, Cell Based Assay |
| BLC-05413P | Human | Human IL-4 Protein, Active, GMP | E.Coli | P05112 (25-153aa) | ≥ 98% | ED50: < 0.2 ng/mL | SDS-PAGE, HPLC, Cell Based Assay |
| BLC-05335P | Human | Human IL-4 Protein, Active | E.coli | P05112 (25-153aa) | ≥ 95% | ED50: < 2 ng/mL | SDS-PAGE, Cell Based Assay |
| BL-1786NP | Human | Human IL-4 Protein (E. coli), Active | E.coli | P05112 (25-153aa) | ≥ 95% | ED50: 10-70 pg/mL | SDS-PAGE, HPLC, Cell Based Assay, BLI |
| BLC-08618P | Human | Human IL-4 Protein (His-SUMO) | E.coli | P05112 (25-153aa) | ≥ 90% | Not Test | SDS-PAGE |
| BLC-08397P | Human | Human IL-4 Protein (His) | E.coli | P05112 (25-153aa) | ≥ 90% | Not Test | SDS-PAGE |
| BLC-04642P | Human | Human IL-4 Protein (His) | Yeast | P05112 (25-153aa) | ≥ 90% | Not Test | SDS-PAGE |
| BL-0279NP | Mouse | Mouse IL-4 Protein (C-6His), Active | Mammalian cell | P07750 (21-140aa) | ≥ 95% | ED50: 0.035 ng/mL | SDS-PAGE, Cell Based Assay |
| BLC-05312P | Mouse | Mouse IL-4 Protein, Active | E.coli | P07750 (23-140aa) | ≥ 95% | ED50: 0.01 ng/mL | SDS-PAGE, Cell Based Assay |
| BLC-05365P | Mouse | Mouse IL-4 Protein, Active | E.coli | P07750 (23-140aa) | ≥ 95% | ED50: 0.01 ng/mL | SDS-PAGE, Cell Based Assay |
| BLPSN-2903 | Mouse | Mouse IL4 Protein, Active | E. coli | P07750 (23-140) | ≥ 90% | ED50: 0.4-4 ng/mL | SDS-PAGE, Cell Based Assay |
| BLPSN-2904 | Mouse | Mouse IL4 Protein (Q136D, Y139D, His Tag), Active | Baculovirus | P07750 (1-140aa, Q136D, Y139D) | ≥ 95% | ED50: 2-10 ng/mL | SDS-PAGE |
| BLC-05310P | Mouse | Mouse IL-4 Protein (His), Active | Mammalian cell | P07750 (21-140aa) | ≥ 95% | ED50: 0.035 ng/mL | SDS-PAGE, Cell Based Assay |
| BLC-09079P | Cynomolgus Monkey | Cynomolgus Monkey IL-4 Protein (His) | E.coli | P79339 (25-153aa) | ≥ 85% | Not Test | SDS-PAGE |
| BLC-03290P | Cynomolgus Monkey | Cynomolgus Monkey IL-4 Protein (His) | Yeast | P79339 (25-153aa) | ≥ 90% | Not Test | SDS-PAGE |
| BLC-05383P | Rhesus Macaque | Rhesus Macaque IL-4 Protein, Active | E.Coli | P51492 (25-153aa) | ≥ 96% | ED50: < 1.0 ng/mL | SDS-PAGE, Cell Based Assay |
| BLC-00873P | Llama | Lama Glama IL-4 Protein (His) | Yeast | Q865X5 (25-133aa) | ≥ 85% | Not Test | SDS-PAGE |
| BLC-06937P | Llama | Lama Glama IL-4 Protein (His) | E.coli | Q865X5 (25-133aa) | ≥ 90% | Not Test | SDS-PAGE |
| BLC-00180P | Golden hamster | Golden hamster IL-4 Protein (His&StrepII) | E.coli | A0A1U7QAD7 (25-147aa) | ≥ 85% | Not Test | SDS-PAGE |
| BLC-00528P | Golden Hamster | Mesocricetus Auratus IL-4 Protein (His) | Yeast | Q60440 (20-147aa) | ≥ 90% | Not Test | SDS-PAGE |
| BLC-02864P | Sheep | Sheep IL-4 Protein (His-B2M&Myc) | E.coli | P30368 (25-135aa) | ≥ 90% | Not Test | SDS-PAGE |
| BLC-04678P | Sheep | Sheep IL-4 Protein (His) | Yeast | P30368 (25-135aa) | ≥ 90% | Not Test | SDS-PAGE |
| BLPSN-2899 | Canine | Canine IL4 Protein, Active | E. coli | O77762 (25-132aa) | ≥ 96% | ED50: < 5-30 ng/mL | SDS-PAGE, Cell Based Assay |
| BLC-10708P | Dog | Dog IL-4 Protein (His) | E.coli | O77762 (25-132aa) | ≥ 90% | Not Test | SDS-PAGE |
Applications of Human Recombinant IL-4
The biological actions of interleukin 4 recombinant human IL-4 make it a versatile cytokine used in both basic and translational research. Some of the most common applications include:
- Immunotherapy development: IL-4 plays a role in anti-tumor immunity and has been evaluated for cancer immunotherapy approaches, especially in combination with other cytokines.
- Allergy and asthma models: Since IL-4 promotes IgE synthesis, it's heavily studied in allergic reaction models.
- Autoimmune disease research: IL-4 is studied for its regulatory effects on inflammatory responses in autoimmune diseases like lupus and multiple sclerosis.
- Stem cell differentiation: Human recombinant IL-4 can modulate stem cell fate, influencing macrophage polarization or dendritic cell development.
- T-cell signaling studies: Researchers use IL-4 to investigate pathways related to Th2 activation and function.
Choosing the Right IL-4 for Your Research
Beta LifeScience offers a wide selection of recombinant IL-4 proteins, produced in different host systems such as mammalian cells, E. coli, yeast, and insect cells. Each variant is selected for optimal compatibility with different applications:
- Mammalian cell-derived IL-4 is ideal for researchers requiring native-like post-translational modifications and high bioactivity.
- Bacterial expression IL-4 offers a cost-effective option for routine assays and high-throughput screening.
- Tagged IL-4 versions (e.g., His-tag, Fc-tag) allow for easy purification and detection during assays.
Regardless of the host system, every recombinant IL-4 product undergoes rigorous quality control to ensure proper folding, purity, and activity.
Advantages of Beta LifeScience’s Recombinant IL-4
We understand the importance of reliability in scientific research. That’s why our recombinant IL-4 proteins are manufactured under stringent quality systems, designed to meet the demands of advanced biomedical research. Here’s what sets our IL-4 proteins apart:
- Purity ≥95% or higher verified by SDS-PAGE and HPLC
- Low endotoxin levels suitable for sensitive cell cultures
- Batch-specific biological activity testing to ensure consistent performance
- Flexible formats (with or without tags) for diverse assay requirements
- Support from experienced scientists for protocol optimization and troubleshooting
Whether you're studying basic cytokine biology or working on translational drug development, Beta LifeScience delivers IL-4 you can trust.
Understanding the Mechanism of IL-4
Recombinant IL-4 binds to the IL-4 receptor complex, which consists of IL-4Rα and the common gamma chain (γc). This interaction activates the JAK-STAT signaling pathway, particularly STAT6, which is responsible for driving the transcription of IL-4 responsive genes.
IL-4 signaling leads to:
- Increased MHC class II expression
- Upregulation of CD23 on B cells
- Enhanced IgE class switching
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Suppression of pro-inflammatory cytokines
Understanding these mechanisms helps researchers unlock how IL-4 can be both protective and pathological, depending on the biological context.
Integrating IL-4 in Experimental Workflows
For researchers planning to add human recombinant IL-4 to their workflows, here are a few practical tips:
- Titration is key: Always optimize the IL-4 concentration for your specific cell type and assay conditions. Effective concentrations vary widely depending on the application.
- Storage recommendations: Store IL-4 proteins at –80°C in small aliquots to avoid repeated freeze-thaw cycles. Lyophilized versions offer greater stability and ease of use.
- Combine with other cytokines: IL-4 often works in synergy with IL-13, IL-10, or GM-CSF in polarization assays, co-culture systems, or cytokine cocktails.
For more detailed experimental recommendations, our team is available to assist you.
Trusted by Scientists Worldwide
Beta LifeScience is proud to support cutting-edge research by offering dependable recombinant cytokines, including recombinant IL-4. Our customers include leading academic institutions, pharmaceutical companies, and biotech firms. We are committed to delivering proteins that meet the highest standards, enabling faster discoveries and reliable results.
Whether you're studying IL-4’s role in adaptive immunity or developing novel therapeutics, you can count on our recombinant human IL-4 proteins for consistency, purity, and performance.
FAQs
What is recombinant human IL-4?
Recombinant human IL-4 is a lab-produced form of the interleukin-4 protein, used to study immune responses, allergic conditions, and therapeutic development.
What are the applications of recombinant IL-4 proteins?
Recombinant IL-4 is used in immunology research, drug screening, cell culture experiments, and inflammation studies to evaluate cytokine signaling.
Are your IL-4 proteins bioactive?
Yes, our recombinant IL-4 proteins are tested for biological activity through cell-based assays, ELISA, and BLI to ensure consistent and reliable results.
What species are available for IL-4 proteins?
We offer IL-4 proteins from human, mouse, rhesus macaque, cynomolgus monkey, dog, sheep, and more to support a wide range of research needs.
How should I store recombinant IL-4 proteins?
Store the proteins at –20°C or –80°C, as specified in the datasheet, and avoid repeated freeze-thaw cycles to maintain stability.
Can I use human recombinant IL-4 for in vivo studies?
While many products are bioactive, always consult the datasheet and regulatory guidelines before using recombinant IL-4 in animal models or therapeutic research.
