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SDS-PAGE analysis of Human LAT (Linker For Activation Of T-Cells Family Member 1) - Recombinant Protein, CAT# BLT-01679P, showing >90% purity under 15% SDS-PAGE (Reduced)

Human LAT (Linker For Activation Of T-Cells Family Member 1) - Recombinant Protein

Molecule: LAT;
Species: Human;
Expression system: E.coli;
Tag: N-6His;
Sequence: 25-221aa;

CAT#: BLT-01679P

Western Blot | Positive WB detected in:Jurkat whole cell lysate, Hela whole cell lysate, HepG2 whole cell lysate(treated with EGF or Pervanadate) | All lanes: Phospho-LAT antibody at 2.9μg/ml | Secondary | Goat polyclonal to rabbit IgG at 1/50000 dilution | Predicted band size: 38 KDa | Observed band size: 38 KDa

IHC image of Phospho-LAT (Y191) Recombinant Monoclonal Antibody diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Phospho-LAT (Y191) Recombinant Monoclonal Antibody

CAT#: BLC-06480A

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