Products H3f3a

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    IHC image of the antibody diluted at 1:100 and staining in paraffin-embedded human pancreatic tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    IHC image of Histone H3.3 Recombinant Monoclonal Antibody diluted at 1:100 and staining in paraffin-embedded human lymph node tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    Histone H3.3 Recombinant Monoclonal Antibody
    CAT#: BLC-06512A
    Western Blot | Positive WB detected in:Hela whole cell lysate,293 whole cell lysate,NIH/3T3 whole cell lysate | All lanes:Phospho-Histone H3 (T3) antibody at 1.41μg/ml | Secondary | Goat polyclonal to rabbit IgG at 1/50000 dilution | Predicted band size: 16 KDa | Observed band size: 16 KDa
    Phospho-Histone H3.3 (T3) Recombinant Monoclonal Antibody
    CAT#: BLC-06523A
    Western blot | All lanes: Histone H3.3 antibody at 2μg/ml + Hela whole cell lysate | Secondary | Goat polyclonal to rabbit IgG at 1/10000 dilution | Predicted band size: 15 kDa | Observed band size: 15 kDa
    Immunohistochemistry of paraffin-embedded human liver cancer using H3F3A Antibody at dilution of 1:100
    Rabbit anti-Human H3F3A Polyclonal Antibody
    CAT#: BLC-06076A
    Western blot | All lanes: Histone H3.3 antibody at 2μg/ml | Lane 1: 293T whole cell lysate | Lane 2: EC109 whlole cell lysate | Secondary | Goat polyclonal to rabbit IgG at 1/1000 dilution | Predicted band size: 15 kDa | Observed band size: 15 kDa
    Immunohistochemistry of paraffin-embedded human kidney tissue using H3F3A Antibody at dilution of 1:100
    Rabbit anti-Human H3F3A Polyclonal Antibody
    CAT#: BLC-06249A
    Greater than 90% as determined by SDS-PAGE.
    Recombinant Human Histone H3.3 (H3F3A) Protein (GST)
    • Molecule: H3-3A;
    • Species: Human;
    • Expression system: E.coli;
    • Tag: GST;
    • Sequence: Protein Fragment;
    CAT#: BLC-08507P

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    Greater than 90% as determined by SDS-PAGE.
    Recombinant Human Histone H3.3 (H3F3A) Protein (His-SUMO)
    • Molecule: H3-3A;
    • Species: Human;
    • Expression system: E.coli;
    • Tag: His-SUMO;
    • Sequence: Protein Fragment;
    CAT#: BLC-09914P

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