Products H2afz

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    Western Blot | Positive WB detected in: HepG2 whole cell lysate | All lanes: H2AFZ antibody at 1.96µg/ml | Secondary | Goat polyclonal to rabbit IgG at 1/50000 dilution | Predicted band size: 14 kDa | Observed band size: 14 kDa
    IHC image of H2AFZ (Ab-4) Antibody diluted at 1:50 and staining in paraffin-embedded human melanoma performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    Rabbit anti-Human H2AFZ Polyclonal Antibody
    CAT#: BLC-00597A
    Immunofluorescence staining of Hela cells with the antibody at 1:5, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
    Chromatin Immunoprecipitation Hela (4*106) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5µg anti-H2AFZ (Mono-methyl-H2AFZ (K4) Antibody) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the β-Globin promoter.
    Rabbit anti-Human H2AFZ Polyclonal Antibody
    CAT#: BLC-00598A
    Western Blot | Positive WB detected in: HepG2 whole cell lysate, A549 whole cell lysate, K562 whole cell lysate, HL60 whole cell lysate | All lanes: H2AFZ antibody at 0.8µg/ml | Secondary | Goat polyclonal to rabbit IgG at 1/50000 dilution | Predicted band size: 14 kDa | Observed band size: 14 kDa
    IHC image of H2AFZ (Ab-7) Antibody diluted at 1:50 and staining in paraffin-embedded human prostate tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    Rabbit anti-Human H2AFZ Polyclonal Antibody
    CAT#: BLC-00601A
    Western Blot | Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate, A549 whole cell lysate, K562 whole cell lysate (All treated by 30mM sodium butyrate for 4h) | All lanes: H2AFZ antibody at 0.48µg/ml | Secondary | Goat polyclonal to rabbit IgG at 1/50000 dilution | Predicted band size: 14 kDa | Observed band size: 14 kDa
    Immunofluorescence staining of Hela cells with Acetyl-H2AFZ (K7) Antibody at 1:25, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
    Rabbit anti-Human H2AFZ Polyclonal Antibody
    CAT#: BLC-00603A
    Western Blot | Positive WB detected in: K562 whole cell lysate, Mouse spleen tissue | All lanes: H2AFZ antibody at 1µg/ml | Secondary | Goat polyclonal to rabbit IgG at 1/50000 dilution | Predicted band size: 14 kDa | Observed band size: 14 kDa
    IHC image of H2AFZ (Ab-4) Antibody diluted at 1:50 and staining in paraffin-embedded human prostate tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    Rabbit anti-Human H2AFZ Polyclonal Antibody
    CAT#: BLC-00604A
    Western Blot | Positive WB detected in: K562 whole cell lysate (treated by 30mM sodium butyrate for 4h) | All lanes: H2AFZ antibody at 1.06µg/ml | Secondary | Goat polyclonal to rabbit IgG at 1/50000 dilution | Predicted band size: 14 kDa | Observed band size: 14 kDa
    Immunofluorescence staining of Hela cells with Acetyl-H2AFZ (K4) Antibody at 1:10, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
    Rabbit anti-Human H2AFZ Polyclonal Antibody
    CAT#: BLC-00605A
    Immunofluorescent analysis of Hela cells (sodium butyrate, 30 mM, 4h) using the antibody at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
    Chromatin Immunoprecipitation Hela (4*106, treated with 30mM sodium butyrate for 4h) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 8µg anti-H2AFZ (Acetyl-H2AFZ (K11) Antibody) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the β-Globin promoter.
    Rabbit anti-Human H2AFZ Polyclonal Antibody
    CAT#: BLC-00615A
    Greater than 90% as determined by SDS-PAGE.
    Recombinant Human Histone H2A.Z (H2AFZ) Protein (GST)
    • Molecule: H2AZ1;
    • Species: Human;
    • Expression system: E.coli;
    • Tag: GST;
    • Sequence: Full Length Protein;
    CAT#: BLC-09306P

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