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Western Blot | Positive WB detected in: Rat spleen tissue | All lanes: H1F0 antibody at 0.85μg/ml | Secondary | Goat polyclonal to rabbit IgG at 1/50000 dilution | Predicted band size: 21, 20 kDa | Observed band size: 21 kDa

IHC image of H1F0 (Ab-101) Antibody diluted at 1:50 and staining in paraffin-embedded human melanoma performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Rabbit anti-Human H1F0 Polyclonal Antibody

CAT#: BLC-00580A

IHC image of the antibody diluted at 1:50 and staining in paraffin-embedded human melanoma performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunofluorescence staining of HepG2 cells with H1F0 (Ab-81) Antibody at 1:2.5, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Rabbit anti-Human H1F0 Polyclonal Antibody

CAT#: BLC-00581A

Western Blot | Positive WB detected in: HepG2 whole cell lysate | All lanes: H1F0 antibody at 1:50 | Secondary | Goat polyclonal to rabbit IgG at 1/50000 dilution | Predicted band size: 21, 20 kDa | Observed band size: 21 kDa

Immunofluorescence staining of HepG2 cells with Mono-methyl-H1F0 (K101) Antibody at 1:2.5, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Rabbit anti-Human H1F0 Polyclonal Antibody

CAT#: BLC-00582A

Immunofluorescence staining of HepG2 cells with the antibody at 1:2.5, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Chromatin Immunoprecipitation Hela (4*106) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5µg anti-H1F0 (Mono-methyl-H1F0 (K81) Antibody) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the β-Globin promoter.

Rabbit anti-Human H1F0 Polyclonal Antibody

CAT#: BLC-00583A

Greater than 90% as determined by SDS-PAGE.

Recombinant Human Histone H1.0 (H1F0) Protein (GST)

Molecule: H1-0;
Species: Human;
Expression system: E.coli;
Tag: GST;
Sequence: Full Length Protein;

CAT#: BLC-09083P

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