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SDS-PAGE analysis of Human CASP4 (Caspase-4) - Recombinant Protein, CAT# BLT-08700P showing >90% purity under 15% SDS-PAGE (Reduced)

Human CASP4 (Caspase-4) - Recombinant Protein

Molecule: CASP4;
Species: Human;
Expression system: E.coli;
Tag: N-6His;
Sequence: 78-265;

CAT#: BLT-08700P

Western Blot | Positive WB detected in: Mouse kidney tissue, Mouse brain tissue, Mouse skeletal muscle tissue | All lanes: CASP4 antibody at 3.5μg/ml | Secondary | Goat polyclonal to rabbit IgG at 1/50000 dilution | Predicted band size: 44, 37, 14, 31, 19 kDa | Observed band size: 44 kDa

IHC image of CASP4 Antibody diluted at 1:700 and staining in paraffin-embedded human pancreatic cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Rabbit anti-Human CASP4 Polyclonal Antibody

CAT#: BLC-01461A

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