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Western Blot | Positive WB detected in: Hela whole cell lysate, Jurkat whole cell lysate, 293 whole cell lysate, MCF-7 whole cell lysate, U251 whole cell lysate, A549 whole cell lysate, HepG2 whole cell lysate | All lanes: AURKB antibody at 1:2000 | Secondary | Goat polyclonal to rabbit IgG at 1/50000 dilution | Predicted band size: 40, 36, 17, 35 kDa | Observed band size: 40 kDa

IHC image of AURKB Recombinant Monoclonal Antibody diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

AURKB Recombinant Monoclonal Antibody

CAT#: BLC-06719A

SDS-PAGE analysis of Human AURKB (Aurora Kinase B) - Recombinant Protein, CAT# BLT-00271P, showing >90% purity under 15% SDS-PAGE (Reduced)

Human AURKB (Aurora Kinase B) - Recombinant Protein

Molecule: AURKB;
Species: Human;
Expression system: E.coli;
Sequence: 1-344aa;

CAT#: BLT-00271P

Immunofluorescence staining of Hela cells with the antibody at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Immunohistochemistry of paraffin-embedded human breast cancer using AURKB Antibody at dilution of 1:100

Rabbit anti-Human AURKB Polyclonal Antibody

CAT#: BLC-05012A

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