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    Western Blot | Positive WB detected in: HepG2 whole cell lysate, MCF-7 whole cell lysate, 293 whole cell lysate, HL60 whole cell lysate, Mouse Heart tissue, Mouse Brain tissue | All lanes: ATP5F1A antibody at 1:2000 | Secondary | Goat polyclonal to rabbit IgG at 1/50000 dilution | Predicted band size: 60, 55, 58 kDa | Observed band size: 60 kDa
    IHC image of ATP5A1 Recombinant Monoclonal Antibody diluted at 1:100 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
    ATP5A1 Recombinant Monoclonal Antibody
    CAT#: BLC-06692A