In Situ Hybridization: Principles, Techniques & Applications

e limited multiplexing capability.

Final Verdict

In situ hybridization remains an essential technique for detecting and localizing nucleic acids within tissues and cells, offering unmatched spatial resolution that other molecular methods cannot provide. Its ability to reveal gene expression patterns, chromosomal changes, and pathogen presence directly in their native context makes it invaluable for both research and clinical diagnostics. While challenges such as technical complexity and cost exist, ongoing advancements—including automation, AI integration, and single-molecule approaches—are continually enhancing its accessibility and precision. As these innovations progress, this method is set to play an even greater role in personalized medicine and advanced biological studies, making it a powerful tool for understanding health and disease at the molecular level.