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Recombinant Human CD14 Protein

Cat No.: BL-0005PS
CD14 Human Recombinant
Description Related products
tag His
host species Human
synonym Monocyte differentiation antigen CD14, Myeloid cell-specific leucine-rich glycoprotein, CD14.
background CD14 (also known lipopolysaccharide (LPS) receptor) is expressed strongly on monocytes and macrophage and weakly on the surface of neutrophils. CD14 is anchored to cells by linkage to glycosylphosphatidylinositol (GPI) and functions as a high affinity receptor for complexes of LPS and LPS binding protein (LBP). Soluble CD14, also binding to LPS, acts at physiological concentration as an LPS agonist and has, at higher concentrations, an LPS antagonizing effect in cell activation. CD14 has been shown to bind apoptotic cells.
description CD14 Human Recombinant expressed in E.Coli is a single, non-glycosylated polypeptide chain containing 353a.a. (20-349 a.a) and having a molecular weight of 37.9kDa. CD14is fused to a 23a.a. His-tag at N-terminus and purified by unique purification methods.
source E.coli
AA sequence MGSSHHHHHH SSGLVPRGSH MGSTTPEPCE LDDEDFRCVC NFSEPQPDWS EAFQCVSAVE VEIHAGGLNL EPFLKRVDAD ADPRQYADTV KALRVRRLTV GAAQVPAQLL VGALRVLAYS RLKELTLEDL KITGTMPPLP LEATGLALSS LRLRNVSWAT GRSWLAELQQ WLKPGLKVLS IAQAHSPAFS CEQVRAFPAL TSLDLSDNPG LGERGLMAAL CPHKFPAIQN LALRNTGMET PTGVCAALAA AGVQPHSLDL SHNSLRATVN PSAPRCMWSS ALNSLNLSFA GLEQVPKGLPAKLRVLDLSC NRLNRAPQPD ELPEVDNLTL DGNPFLVPGT ALPHEGSMNS GVV.
purity >90% as determined by SDS-PAGE.
endotoxin <1.0 EU per μg by the LAL method.
formulation CD14 protein solution (0.5mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 30% glycerol 0.15M NaCl and 1mM DTT.
stability Recombinant protein is stable for 12 months at -70℃
usage For Research Use Only
storage Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
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