Recombinant Rat EphB3 Protein (ECD, His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-1838
Recombinant Rat EphB3 Protein (ECD, His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-1838
Catalog No.: BLPSN-1838
Product Overview
| Tag | His |
| Host Species | Rat |
| Accession | NP_001099338.1 |
| Background | Ephrin type-B receptor 3, also known as EphB3 or HEK2, belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family which 16 known receptors (14 found in mammals) are involved: EPHA1, EPHA2, EPHA3, EPHA4, EPHA5, EPHA6, EPHA7, EPHA8, EPHA9, EPHA1, EPHB1, EPHB2, EPHB3, EPHB4, EPHB5, EPHB6. The Eph family of receptor tyrosine kinases (comprising EphA and EphB receptors) has been implicated in synapse formation and the regulation of synaptic function and plasticity6. Ephrin receptors are components of cell signalling pathways involved in animal growth and development, forming the largest sub-family of receptor tyrosine kinases (RTKs). Ligand-mediated activation of Ephs induce various important downstream effects and Eph receptors have been studied for their potential roles in the development of cancer. EphB receptor tyrosine kinases are enriched at synapses, suggesting that these receptors play a role in synapse formation or function. We find that EphrinB binding to EphB induces a direct interaction of EphB with NMDA-type glutamate receptors. This interaction occurs at the cell surface and is mediated by the extracellular regions of the two receptors, but does not require the kinase activity of EphB. |
| Description | A DNA sequence encoding the rat Ephb3 (NP_001099338.1) (Met1-Thr537) was expressed with a His tag at the C-terminus. |
| Source | HEK293 |
| Predicted N Terminal | Leu 30 |
| AA Sequence | Met1-Thr537 |
| Molecular Weight | The recombinant rat Ephb3 consists 519 a.a. and predicts a molecular mass of 57 kDa. |
| Purity | >95% as determined by SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg protein as determined by the LAL method. |
| Bioactivity | Measured by its binding ability in a functional ELISA.Immobilized rat EPHB3-His at 10 ug/ml (100 ul /well) can bind rat EFNB1-Fc, The EC50 of rat EFNB1-Fc is 2-6ng/ml. |
| Formulation | Lyophilized from sterile PBS, pH 7.4.. |
| Stability | The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. |
| Usage | For Research Use Only |
| Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
