Recombinant Mouse Nectin-2 / NECT2 Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-3454
Recombinant Mouse Nectin-2 / NECT2 Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-3454
Our products are highly customizable to meet your specific needs. You can choose options such as endotoxin removal, liquid or lyophilized forms, preferred tags, and the desired functional sequence range for proteins. Submitting a written inquiry expedites the quoting process.
Product Overview
Tag | His |
Host Species | Mouse |
Accession | NP_033016.3 |
Synonym | AI325026, AI987993, Cd112, MPH, nectin-2, Pvr, Pvs |
Background | Cluster of Differentiation 112 (CD112), also known as poliovirus receptor related protein 2 (PVRL2 or PRR2), is a single-pass type I transmembrane glycoprotein belonging to the Immunoglobulin superfamily. CD112 protein also serves as an entry for certain mutant strains of herpes simplex virus and pseudorabies virus, and thus is involved in cell to cell spreading of these viruses. CD112 protein has been identified as the ligand for DNAM-1 (CD226), and the interaction of CD226/CD112 protein can induce NK cell- and CD8+ T cell-mediated cytotoxicity and cytokine secretion. CD112 has been regarded as a critical component in allergic reactions, and accordingly may function as a novel target for anti-allergic therapy. |
Description | A DNA sequence encoding the mouse PVRL2 (NP_033016.3) extracellular domain (Met 1-Gly 351) was fused with a His tag at the C-terminus. |
Source | HEK293 |
Predicted N Terminal | Gln 32 |
AA Sequence | Met 1-Gly 351 |
Molecular Weight | The secreted recombinant mouse PVRL2 comprises 331 a.a. with a predicted molecular mass of 36 kDa. As a result of glycosylation, it migrates as an approximately 40-45 kDa band in SDS-PAGE under reducing conditions. |
Purity | >98% as determined by SDS-PAGE |
Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method |
Bioactivity | Measured by its binding ability in a functional ELISA.1. Immobilized mouse CD112-His at 10 ug/ml (100 ul/well) can bind biotinylated mouse DNAM1-His with a linear range of 0.156-5.0 ug/ml.2. Immobilized mouse CD112-His at 10 ug/ml (100 ul/well) can bind mouse DNAM1-Fc with a linear range of 0.03-1.0 ug/ml. |
Formulation | Lyophilized from sterile PBS, pH 7.4. |
Stability | The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. |
Usage | For Research Use Only |
Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |