Recombinant Mouse EphB3 Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-1837
Recombinant Mouse EphB3 Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-1837
Collections: Enzymes, Kinase, Recombinant proteins
Our products are highly customizable to meet your specific needs. You can choose options such as endotoxin removal, liquid or lyophilized forms, preferred tags, and the desired functional sequence range for proteins. Submitting a written inquiry expedites the quoting process.
Product Overview
Tag | His |
Host Species | Mouse |
Accession | NP_034273.1 |
Synonym | AW456895, Cek10, Etk2, HEK2, MDK5, Sek4, Tyro6 |
Background | Ephrin type-B receptor 3, also known as EphB3 or HEK2, belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family which 16 known receptors (14 found in mammals) are involved: EPHA1, EPHA2, EPHA3, EPHA4, EPHA5, EPHA6, EPHA7, EPHA8, EPHA9, EPHA1, EPHB1, EPHB2, EPHB3, EPHB4, EPHB5, EPHB6. The Eph family of receptor tyrosine kinases (comprising EphA and EphB receptors) has been implicated in synapse formation and the regulation of synaptic function and plasticity6. Ephrin receptors are components of cell signalling pathways involved in animal growth and development, forming the largest sub-family of receptor tyrosine kinases (RTKs). Ligand-mediated activation of Ephs induce various important downstream effects and Eph receptors have been studied for their potential roles in the development of cancer. EphB receptor tyrosine kinases are enriched at synapses, suggesting that these receptors play a role in synapse formation or function. We find that EphrinB binding to EphB induces a direct interaction of EphB with NMDA-type glutamate receptors. This interaction occurs at the cell surface and is mediated by the extracellular regions of the two receptors, but does not require the kinase activity of EphB. |
Description | A DNA sequence encoding the mouse EPHB3 (NP_034273.1) extracellular domain (Met 1-Thr 537) was expressed, fused with a His tag at the C-terminus. |
Source | HEK293 |
Predicted N Terminal | Leu 30 |
AA Sequence | Met 1-Thr 537 |
Molecular Weight | The secreted recombinant mouse EPHB3 consists of 519 a.a. and has a predicted molecular mass of 57 kDa. It migrates as an approximately 63 kDa band in SDS-PAGE under reducing conditions. |
Purity | >94% as determined by SDS-PAGE |
Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method |
Bioactivity | Measured by its binding ability in a functional ELISA. Immobilized mouse EphB3 at 2 ug/ml (100 ul/well) can bind mouse EFNB1 with a linear range of 0.1-12.5 ng/ml. |
Formulation | Lyophilized from sterile 20mM Tris, 150mM NaCl, pH 7.5. |
Stability | The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. |
Usage | For Research Use Only |
Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |