Recombinant Mouse EphA6 Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-1817
Recombinant Mouse EphA6 Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-1817
Catalog No.: BLPSN-1817
Product Overview
| Tag | His |
| Host Species | Mouse |
| Accession | NP_031964.2 |
| Synonym | Ehk2, Hek12, m-ehk2 |
| Background | Ephrin type-A receptor 6, also known as EphA6 or EHK2, belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family which 16 known receptors (14 found in mammals) are involved: EPHA1, EPHA2, EPHA3, EPHA4, EPHA5, EPHA6, EPHA7, EPHA8, EPHA9, EPHA1, EPHB1, EPHB2, EPHB3, EPHB4, EPHB5, EPHB6. The Eph family of receptor tyrosine kinases (comprising EphA and EphB receptors) has been implicated in synapse formation and the regulation of synaptic function and plasticity6. Eph receptor-mediated signaling, which is triggered by ephrins7, probably modifies the properties of synapses during synaptic activation and remodeling. Ephrin receptors are components of cell signalling pathways involved in animal growth and development, forming the largest sub-family of receptor tyrosine kinases (RTKs). Ligand-mediated activation of Ephs induce various important downstream effects and Eph receptors have been studied for their potential roles in the development of cancer. In the vomeronasal system, Ephrin-A5/EphA6 interactions mediate attraction or adhesion rather than repulsion. |
| Description | A DNA sequence encoding the extracellular domain of mouse EphA6 (NP_031964.2) (Met 1-Gln 546) was expressed, with a C-terminal His tag. |
| Source | HEK293 |
| Predicted N Terminal | Ser 28 |
| AA Sequence | Met 1-Gln 546 |
| Molecular Weight | The secreted recombinant mouse EphA6 comprises 530 a.a. and has a calculated molecular mass of 59.5 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 65 kDa band in SDS-PAGE under reducing conditions. |
| Purity | >97% as determined by SDS-PAGE |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method |
| Bioactivity | Measured by its binding ability in a functional ELISA. Immobilized recombinant mouse EphA6 at 2 ug/ml (100 ul/well) can bind recombinant human EphrinA3 at a linear range of 0.31-10 ng/ml. |
| Formulation | Lyophilized from sterile PBS, pH 7.4. |
| Stability | The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. |
| Usage | For Research Use Only |
| Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
