Recombinant Mouse Carboxypeptidase M Protein (His Tag)

Beta LifeScience SKU/CAT #: BLPSN-0577
Recombinant Mouse Carboxypeptidase M Protein (His Tag)

Recombinant Mouse Carboxypeptidase M Protein (His Tag)

Beta LifeScience SKU/CAT #: BLPSN-0577
Catalog No.: BLPSN-0577

Product Overview

Tag His
Host Species Mouse
Accession Q80V42
Synonym 1110060I01Rik, 5730456K23Rik, AA589379, E030045M14Rik
Background Carboxypeptidase M, also known as CPM, is a membrane-bound arginine/lysine carboxypeptidase which is a member of the carboxypeptidases family. These enzymes remove C-terminal amino acids from peptides and proteins and exert roles in the physiological processes of blood coagulation/fibrinolysis, inflammation, food digestion and pro-hormone and neuropeptide processing. Among the carboxypeptidases CPM is of particular importance because of its constitutive expression in an active form at the surface of specialized cells and tissues in the human body. CPM in the brain appears to be membrane-bound via a phosphatidylinositol glycan anchor. CPM is widely distributed in a variety of tissues and cells. The amino acid sequence of CPM indicated that the C-terminal hydrophobic region might be a signal for membrane attachment via a glycosylphosphatidylinositol (GPI) anchor. CPM is involved in peptide metabolism on both the cell surface and in extracellular fluids. CPM functions not only as a protease but also as a binding partner in cell-surface protein-protein interactions.
Description A DNA sequence encoding the extracellular domain of mouse CPM isoform 1 (Q80V42-1) (Met 1-His 422), without the propeptide, was fused with a C-terminal His tag.
Source HEK293
Predicted N Terminal Leu 18
AA Sequence Met 1-His 422
Molecular Weight The secreted recombinant mouse CPM comprises 416 a.a. and has a calculated molecular mass of 47.8 kDa. The apparent molecular mass of rmCPM is approximately 52 kDa in SDS-PAGE under reducing conditions.
Purity >97% as determined by SDS-PAGE
Endotoxin < 1.0 EU per μg of the protein as determined by the LAL method
Bioactivity Measured by its ability to release Larginine from BenzoylAlaArg, with detection of the arginine amino group by ophthaldialdehyde.The specific activity is >40,000 pmoles/min/ug.
Formulation Lyophilized from sterile PBS, pH 7.4.
Stability The recombinant proteins are stable for up to 1 year from date of receipt at -70°C.
Usage For Research Use Only
Storage Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Recently viewed