Recombinant Mouse ALK-1 Protein (His & Fc Tag)
Beta LifeScience
SKU/CAT #: BLPSN-0139
Recombinant Mouse ALK-1 Protein (His & Fc Tag)
Beta LifeScience
SKU/CAT #: BLPSN-0139
Catalog No.: BLPSN-0139
Product Overview
| Tag | His&Fc |
| Host Species | Mouse |
| Accession | NP_033742.2 |
| Synonym | Acvrlk1, AI115505, AI427544, Alk1 |
| Background | Activin A receptor, type II-like 1 (ACVRL1), also known as ALK-1 (activin receptor-like kinase 1), is an endothelial-specific type I receptor of the TGF-beta (transforming growth factor beta) receptor family of ligands. On ligand binding, a heteromeric receptor complex forms consisting of two type II and two type I transmembrane serine/threonine kinases. ACVRL1 protein is expressed in certain blood vessels of kidney, spleen, heart and intestine, serving as an important role during vascular development. Mutations in ACVRL1 gene are associated with hemorrhagic telangiectasia type 2, also known as Rendu-Osler-Weber syndrome 2 and vascular disease. |
| Description | A DNA sequence encoding the mouse ALK1 (NP_033742.2) precursor (Met 1-Pro 119) was fused with the C-terminal His-tagged Fc region of human IgG1 at the C-terminus. |
| Source | HEK293 |
| Predicted N Terminal | Asp 23 |
| AA Sequence | Met 1-Pro 119 |
| Molecular Weight | The secreted recombinant mouse ALK1/Fc is a disulfide-linked homodimer after removal of the signal peptide. The reduced monomer comprises 345 a.a. with a predicted molecular mass of 39 kDa. As a result of glycosylation, it migrates as an approximately 50-55 kDa band in SDS-PAGE under reducing conditions. |
| Purity | >95% as determined by SDS-PAGE |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method |
| Bioactivity | Measured by its ability to inhibit BMP9-induced alkaline phosphatase production by MC3T3E1 mouse chondrogenic cells. David, L. et al. (2007) Blood 109:1953.The ED50 for this effect is typically 60-300 ng/mL in the presence of 2 ng/mL of recombiant human BMP9. |
| Formulation | Lyophilized from sterile PBS, pH 7.4. |
| Stability | The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. |
| Usage | For Research Use Only |
| Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
