Recombinant Mouse ADAM15 Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-0085
Recombinant Mouse ADAM15 Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-0085
Catalog No.: BLPSN-0085
Product Overview
| Tag | His |
| Host Species | Mouse |
| Accession | O88839 |
| Synonym | MDC15, metargidin |
| Background | ADAM15, also known as Metargidin, is a type I transmembrane glycoprotein belonging to the ADAM (A Disintegrin and Metalloprotease Domain) family of proteins and is widely expressed in different tissues and cell types. Members of this family contain an amino-terminal metalloprotease domain followed by a disintegrin domain, a cysteine-rich region and a membrane proximal EGF-like domain. The disintegrin domain of ADAM15/metargidin contains an RGD tripeptide sequence, suggesting that it may potentially interact with the integrin family of proteins. ADAM15 is a transmembrane multi-domain proteins implicated in proteolysis, cell-cell and cell-matrix interactions in various disease conditions. There is also evidence supporting a role for ADAM15 in angiogenesis and angioinvasion of tumor cells, which are critical for unrestrained tumor growth and metastatic spread. Given its diverse functions, ADAM15 may represent a pivotal regulatory component of tumor progression, an important target for therapeutic intervention, or emerge as a biomarker of disease progression. |
| Description | A DNA sequence encoding the mouse ADAM15 (O88839-1) extracellular domain (Met 1-Thr 697) was expressed, with a C-terminal His tag. |
| Source | HEK293 |
| Predicted N Terminal | Arg 18 & Asp 208 |
| AA Sequence | Met 1-Thr 697 |
| Molecular Weight | The secreted recombinant mouse ADAM15 (pro form) comprises 691 a.a. and has a calculated molecular mass of 74.6 kDa. As a result of glycosylation, the apparent molecular mass of the recombinant protein is approximately 80-90 kDa in SDS-PAGE under reducing conditions. |
| Purity | >90% as determined by SDS-PAGE |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method |
| Bioactivity | 1. Measured by its ability to bind human PTK6 in a functional ELISA.2. Measured by its ability to bind human SRC in a functional ELISA.3. Measured by its ability to bind mouse SRC in a functional ELISA. 4. Measured by its ability to bind human LYN in a functional ELISA. |
| Formulation | Lyophilized from sterile PBS, pH 7.4. |
| Stability | The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. |
| Usage | For Research Use Only |
| Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
