Recombinant Human XIAP Protein (AVI Tag)
Beta LifeScience
SKU/CAT #: BLPSN-4862
Recombinant Human XIAP Protein (AVI Tag)
Beta LifeScience
SKU/CAT #: BLPSN-4862
Catalog No.: BLPSN-4862
Product Overview
| Tag | AVI |
| Host Species | Human |
| Accession | NP_001158.2 |
| Synonym | API3, BIRC4, hIAP-3, hIAP3, IAP-3, ILP1, MIHA, XLP2 |
| Background | E3 ubiquitin-protein ligase XIAP / BIRC4, also known as inhibitor of apoptosis protein 3, X-linked inhibitor of apoptosis protein, and IAP-like protein, is a protein that belongs to a family of apoptotic suppressor proteins. Members of this family share a conserved motif termed, baculovirus IAP repeat, which is necessary for their anti-apoptotic function. XIAP / BIRC4 functions through binding to tumor necrosis factor receptor-associated factors TRAF1 and TRAF2 and inhibits apoptosis induced by menadione, a potent inducer of free radicals, and interleukin 1-beta converting enzyme. XIAP / BIRC4 also inhibits at least two members of the caspase family of cell-death proteases, caspase-3 and caspase-7. Mutations in this encoding gene are the cause of X-linked lymphoproliferative syndrome. Alternate splicing results in multiple transcript variants. Thought to be the most potent apoptosis suppressor, XIAP / BIRC4, directly binds and inhibits caspases -3, -7 and -9. Survivin, which also binds to several caspases, is up-regulated in a many tumour cell types. Defects in XIAP / BIRC4 are the cause of lymphoproliferative syndrome X-linked type 2 (XLP2). XLP is a rare immunodeficiency characterized by extreme susceptibility to infection with Epstein-Barr virus (EBV). Symptoms include severe or fatal mononucleosis, acquired hypogammaglobulinemia, pancytopenia and malignant lymphoma. |
| Description | A DNA sequence encoding the human XIAP (NP_001158.2) (Leu 121-Thr 356) was fused with an AVI tag at the C-terminus, and additonal two amino acids (Gly & Pro) at the N-terminus. |
| Source | E.coli |
| Predicted N Terminal | Met |
| AA Sequence | Leu 121-Thr 356 |
| Molecular Weight | The recombinant human XIAP comprises 253 a.a. and has a predicted molecular mass of 29.1 kDa as estimated in SDS-PAGE under non-reduced conditions. |
| Purity | >75% as determined by SDS-PAGE |
| Endotoxin | Please contact us for more information. |
| Bioactivity | Measured by its binding ability in a functional ELISA.Immobilized recombinant human SMAC-His can bind recombinant human XIAP-AVI with a linear range of 0.125-1.0 ug/ml. |
| Formulation | Lyophilized from sterile 25mM Tris, 10mM DTT, 1% glycerol, 0.2M Glutamine Potassium, pH 8.0. |
| Stability | The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. |
| Usage | For Research Use Only |
| Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
