Recombinant Human Ras Association Domain-Containing Protein 5 (RASSF5) Protein (GST)

Name: Recombinant Human Ras Association Domain-Containing Protein 5 (RASSF5) Protein (GST)
Brand: Beta LifeScience
SKU: BLC-08791P
Availability: InStock

Greater than 90% as determined by SDS-PAGE.

Collections: High-quality recombinant proteins, Other recombinant proteins

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Product Overview

Description	Recombinant Human Ras Association Domain-Containing Protein 5 (RASSF5) Protein (GST) is produced by our E.coli expression system. This is a full length protein.
Purity	Greater than 90% as determined by SDS-PAGE.
Uniprotkb	Q8WWW0
Target Symbol	RASSF5
Synonyms	Maxp 1; MAXP1; MGC10823; MGC17344; New ras effector 1; NORE 1; NORE1; NORE1A; NORE1B; Novel Ras effector 1; RAP1 Binding Protein; RAPL; Ras association (RalGDS/AF 6) domain family 5; Ras association (RalGDS/AF 6) domain family member 5; Ras association domain containing family protein 5; Ras association domain-containing protein 5; Ras effector like protein; RASF5_HUMAN; RASSF 3; RASSF 5; RASSF3; RASSF5; Regulator for cell adhesion and polarization enriched in lymphoid tissue; Regulator for cell adhesion and polarization enriched in lymphoid tissues; Tumor suppressor RASSF3
Species	Homo sapiens (Human)
Expression System	E.coli
Tag	N-GST
Target Protein Sequence	MTVDSSMSSGYCSLDEELEDCFFTAKTTFFRNAQSKHLSKNVCKPVEETQRPPTLQEIKQKIDSYNTREKNCLGMKLSEDGTYTGFIKVHLKLRRPVTVPAGIRPQSIYDAIKEVNLAATTDKRTSFYLPLDAIKQLHISSTTTVSEVIQGLLKKFMVVDNPQKFALFKRIHKDGQVLFQKLSIADRPLYLRLLAGPDTEVLSFVLKENETGEVEWDAFSIPELQNFLTILEKEEQDKIQQVQKKYDKFRQKLEEALRESQGKPG
Expression Range	1-265aa
Protein Length	Full Length of Isoform 2
Mol. Weight	57.4kDa
Research Area	Cell Biology
Form	Liquid or Lyophilized powder
Buffer	Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution	Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	Potential tumor suppressor. Seems to be involved in lymphocyte adhesion by linking RAP1A activation upon T-cell receptor or chemokine stimulation to integrin activation. Isoform 2 stimulates lymphocyte polarization and the patch-like distribution of ITGAL/LFA-1, resulting in an enhanced adhesion to ICAM1. Together with RAP1A may participate in regulation of microtubule growth. The association of isoform 2 with activated RAP1A is required for directional movement of endothelial cells during wound healing. May be involved in regulation of Ras apoptotic function. The RASSF5-STK4/MST1 complex may mediate HRAS and KRAS induced apoptosis.
Subcellular Location	Cytoplasm. Cytoplasm, cytoskeleton. Note=Isoform 2 is mainly located in the perinuclear region of unstimulated primary T-cells. Upon stimulation translocates to the leading edge and colocalizes with ITGAL/LFA-1 in the peripheral zone of the immunological synapse. Isoform 2 is localized to growing microtubules in vascular endothelial cells and is dissociated from microtubules by activated RAP1A.
Database References	HGNC: 17609 OMIM: 607020 KEGG: hsa:83593 STRING: 9606.ENSP00000347443 UniGene: PMID: 29127148 Hepatitis C virus uses NS5B to specifically suppress NORE1A, facilitating viral replication and elevated Ras signaling. PMID: 28090674 Our study demonstrated that miR-214 expression was elevated and RASSF5 was down regulated in oral cancer. Moreover, miR-214 suppressed KB cell apoptosis through down regulation of RASSF5 expression PMID: 28290615 mCD40L-induced cell death mediated by NORE1A expression appeared to be independent of mCD40L-induced cell death mediated by sustained JNK activation since NORE1A inhibition did not affect JNK phosphorylation and vice versa PMID: 26986513 Ras induces the formation of a complex between NORE1A and the phosphatase PP1A, promoting the activation of the Rb tumor suppressor by dephosphorylation PMID: 26677227 Down-regulation of RASSF5A and RASSF5C expression is a tumor-specific phenomenon. PMID: 25420558 RASSF5 expression is negatively correlated with distant metastasis of osteosarcoma, and RASSF5 may function as a tumor suppressor in OS cells through activation of the MST1/LATS1 pathway. PMID: 25109282 NORE1A allows Ras to qualitatively modify p53 function to promote senescence. PMID: 25778922 the inactivation of RASSF5A through CpG island 1 methylation may play an important role in esophageal squamous cell carcinoma (ESCC) carcinogenesis, RASSF5A may be a functional tumor suppressor and may serve as a prognostic biomarker for ESCC. PMID: 25579665 NORE1A has a role in Ras regulation of SCF(beta-TrCP) protein activity and specificity PMID: 25217643 RASSF5 can act as an inhibitor or a potential positive regulator of Mst2, depending on whether it binds to Mst2 before or after activation-loop phosphorylation. PMID: 23972470 Ubiquitin ligase Itch is a unique negative regulator of RASSF5. PMID: 23538446 The RASSF gene family members RASSF5, RASSF6 and RASSF7 show frequent DNA methylation in neuroblastoma. PMID: 22695170 crystal of NORE1 diffracted to 2.7 A resolution and belonged to space group P6(1)22, with unit-cell parameters a = b = 73.041, c = 66.092 A, alpha = beta = 90, gamma = 120 degrees PMID: 22750872 These findings indicate that the control of HIPK1 stability by Mdm2-NORE1 has a major effect on cell behaviour, and epigenetic inactivation of NORE1 enables adenocarcinoma formation in vivo through HIPK1 stabilization. PMID: 22173032 NORE1A has activity that suppresses the centrosome amplification induced by HU and that NORE1A mRNA down-regulation is one of the common gene abnormalities in NSCLCs, both of which imply a key preventive role of NORE1A against the carcinogenesis of NSCLC. PMID: 20434789 epigenetic inactivation of NORE1 due to aberrant promoter hypermethylation is a frequent event in colorectal tumorigenesis and might be implicated in the malignant progression of colorectal tumors PMID: 20969767 a novel regulatory network composed of the tumor suppressor NORE1A, the mitotic kinase Aurora A, the small GTPase Ras, and the microtubule cytoskeleton. PMID: 20339001 findings define a T cell receptor "inside-out" pathway via N-SKAP1-C-RapL that regulates T cell adhesion, motility, and arrest times with dendritic cells in lymph nodes. PMID: 20346707 The present investigation provided evidence that Lck-mediated phosphorylation regulates the nucleocytoplasmic shuttling and cell growth control activities of RASSF5. PMID: 20064523 Nore1 is a member of a family of Ras effector/tumor suppressors that includes RASSF1 PMID: 12676952 the epigenetic alteration of NORE1A is confined to lung tumors with a wild-type K-ras: 88% (15 of 17) of the tumors with NORE1 hypermethylation did not harbor a K-ras mutation (P=0.008, Fisher's exact test) PMID: 15378027 Rap1 activation contributes to directional vascular endothelial cell migration accompanied by extension of microtubules on which RAPL localizes PMID: 15569673 results indicate that RASSF1A epigenetic changes are an early event in thyroid tumor pathogenesis and progression PMID: 15980887 Suppression of NORE1A, a known Ras effector, in PAX8-PPARgamma fusion-carrying follicular thyroid cancer. PMID: 16352687 association of NORE1A with cytoskeletal elements is essential for NORE1A-induced growth suppression and that the ERK pathway is a target for NORE1A growth-suppressive activities PMID: 16421102 The candidate tumor suppressor gene NORE1B is epigenetically down-regulated in hepatocellular carcinoma. PMID: 16516329 These findings suggest that endogenous Nore1B recruits active Ras to the APC-T cell interface and mediates the interaction between Ras and Carma1. PMID: 16520020 The NORE1A gene was never found epigenetically methylated in hepatitic or non-hepatitic liver. PMID: 16606445 Nuclear localization of RASSF5 is critical for its cell growth control activity. PMID: 17320110 interaction surfaces in RAPL-Rap1 and RAPL-Rap2 complexes are different and that a single residue in the switch I region of Rap proteins (residue 39) contributes considerably to the different kinetics of these protein-protein interactions. PMID: 17716979 nuclear export of NORE1A via nuclear export signal is involved in the NORE1A-mediated induction of apoptosis PMID: 18211824 NORE1A promoter methylation is a rare event in neuroblastoma cells and primary tumors. Other mechanisms are likely to account for the frequent reduction of NORE1A mRNA expression. Also, antitumorigenic role of NORE1A in human neuroblastoma is evident. PMID: 18452173 Study describes the crystal structure of Ras in complex with the Ras binding domain (RBD) of NORE1A; the contact area of NORE1A is extended as compared with other Ras effectors & provides a rationale for an exceptionally long lifetime of the complex. PMID: 18596699 findings showed that only 4% of the glioma tumors revealed a methylated promoter for NORE1A PMID: 18616639 Data show that degradation by calpains is a novel mechanism for downregulation of NORE1A and RASSF1A proteins and might be the mechanism allowing cancer cells to escape growth suppression. PMID: 19098985 NORE1B suppresses replication and transformation of cells as effectively as RASSF1A and is a putative tumor suppressor gene. NORE1B interacts with RASSF1A and loss of one of these may lead to uncontrolled growth and transformation of hepatocytes. PMID: 19118008 NORE1A activates p21(CIP1) via promoting p53 nuclear localization. PMID: 19435914

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

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