Recombinant Human NPM1 & ALK (F1174L) Protein

Beta LifeScience SKU/CAT #: BL-0252SG
Recombinant Human NPM1 & ALK (F1174L) Protein

Recombinant Human NPM1 & ALK (F1174L) Protein

Beta LifeScience SKU/CAT #: BL-0252SG
Catalog No.: BL-0252SG

Product Overview

Tag GST
Host Species Human
Accession U04946
Synonym NPM1: B23; NPMALK: ALK (Ki-1), CD246, NBLST3, TFG/ALK
Background NPM1-ALK which belongs to the insulin receptor superfamily which comprises an extracellular domain, a hydrophobic stretch corresponding to a single pass transmembrane region, and an intracellular kinase domain. CD246 plays an important role in the development of the brain and exerts its effects on specific neurons in the nervous system. ALK-positive neoplasms represent a distinct entity because the morphology of the tumors is often neither anaplastic nor large cell and the tumors should be referred to as ALK lymphomas (1). CD246 has been found to be rearranged, mutated, or amplified in a series of tumours including anaplastic large cell lymphomas, neuroblastoma, and non-small cell lung cancer. STAT5A is epigenetically silenced by the tyrosine kinase NPM1-ALK and acts as a tumor suppressor by reciprocally inhibiting NPM1-ALK expression (2).
Description Recombinant human fusion protein NPM1 (1-117)-ALK (F1174L) (1058-end) was produced by baculovirus in Sf9 insect cells, fused with a GST tag at N-terminus. This protein is purified with our unique purification methods.
Source Sf9 insect cells
AA Sequence 1058a.a.-end
Molecular Weight ~108 kDa
Purity For specific purity information on a given lot, see related COA.
Endotoxin < 1.0 EU per μg of the protein as determined by the LAL method
Bioactivity Active
Formulation Recombinant protein is supplied in 50mM Tris-HCl, pH 7.5, 50mM NaCl, 10mM Glutathione, 0.25mM DTT, 0.1mM EDTA, 0.1mM PMSF and 25% glycerol.
Stability The recombinant protein is stable for up to 12 months at -70°C
Usage For Research Use Only
Storage Recombinant Human NPM1 & ALK (F1174L) Protein should be stored should be stored at < -70°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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