Recombinant Human NKp30 Protein (His Tag)

Name: Recombinant Human NKp30 Protein (His Tag)
Brand: Beta LifeScience
SKU: BLPSN-3523
Availability: InStock

Collections: Co-stimulatory receptors, Other recombinant proteins, Recombinant proteins

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Product Overview

Tag	His
Host Species	Human
Accession	NP_667341.1
Synonym	1C7, CD337, DAAP-90L16.3, LY117, MALS, NCR3, NKp30
Background	Natural Cytotoxicity Triggering Receptor 3, NCR3, also known as NKp3, or CD337, is a natural cytotoxicity receptor, expressed on subsets of human peripheral blood NK cells, involved in NK cell killing of tumor cells and immature dendritic cells. The cellular ligand for NKp3 has remained elusive, but the membrane-associated heparan sulfate (HS) proteoglycans are involved in the recognition of cellular targets by NKp3 was recently reported. NKp3 is a member of the immunoglobulin superfamily and one of three existing natural cytotoxicity-triggering receptors. NKp3 is a glycosylated protein and is thought to be selectively expressed in resting and activated natural killer cells. NKp3 is a stimulatory receptor on human NK cells implicated in tumor immunity, and is capable of promoting or terminating dendritic cell maturation. NCR3 may play a role in inflammatory and infectious diseases.
Description	A DNA sequence encoding the extracellular domain (Met 1-Gly 135) of human NCR3 (NP_667341.1) precursor was fused with a His tag at the C-terminus.
Source	HEK293
Predicted N Terminal	Leu 19
AA Sequence	Met 1-Gly 135
Molecular Weight	The recombinant human NCR3 consists of 128 a.a. after removal of the signal peptide and has a predicted molecular mass of 14.4 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rh NCR3 is approximately 22-28 kDa due to glycosylation.
Purity	>97% as determined by SDS-PAGE
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Bioactivity	1.Measured by its binding ability in a functional ELISA. 2.Immobilized human NCR3 at 10ug/mL (100uL/well) can bind human B7-H6 (16140-H02H), the EC50 of human B7-H6 is 20-100ng/mL.
Formulation	Lyophilized from sterile PBS, pH 7.4.
Stability	The recombinant proteins are stable for up to 1 year from date of receipt at -70°C.
Usage	For Research Use Only
Storage	Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.