Recombinant Human INPP5A Protein (C-6His)

Name: Recombinant Human INPP5A Protein (C-6His)
Brand: Beta LifeScience
SKU: BL-1593NP
Availability: InStock

BL-1593NP: Greater than 95% as determined by reducing SDS-PAGE. (QC verified)

Collections: Other recombinant proteins, Recombinant proteins

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Product Overview

Description	Recombinant Human Type I Inositol-polyphosphate Phosphatase is produced by our Mammalian expression system and the target gene encoding Met1-Val410 is expressed with a 6His tag at the C-terminus.
Accession	Q14642
Synonym	Type I inositol 1;4;5-trisphosphate 5-phosphatase; INPP5A
Gene Background	Type I inositol 1,4,5-trisphosphate 5-phosphatase is a 412 amino acids protein that belongs to the inositol 1,4,5-trisphosphate 5-phosphatase type I family. Type I inositol-1,4,5-trisphosphate 5-phosphatase is an enzyme that in humans is encoded by the INPP5A gene. It is expressed in brain and high level in Purkinje cells. InsP3 5-phosphatases hydrolyze Ins(1,4,5)P3, which mobilizes intracellular calcium and acts as a second messenger mediating cell responses to various stimulation.
Molecular Mass	48.7 KDa
Apmol Mass	45 KDa, reducing conditions
Formulation	Supplied as a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.4.
Endotoxin	Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test.
Purity	Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
Biological Activity	Not tested
Reconstitution
Storage	Store at ≤-70°C, stable for 6 months after receipt. Store at ≤-70°C, stable for 3 months under sterile conditions after opening. Please minimize freeze-thaw cycles.
Shipping	The product is shipped on dry ice/polar packs. Upon receipt, store it immediately at the temperature listed below.
Usage	For Research Use Only

Target Details

Target Function	Phosphatase that specifically hydrolyzes the 5-phosphate of inositol 1,4,5-trisphosphate to inositol 1,4-bisphosphate, and inositol 1,3,4,5-tetrasphosphate to inositol 1,3,4-trisphosphate. Plays a crucial role in the survival of cerebellar Purkinje cells.
Subcellular Location	Cell membrane; Lipid-anchor. Cell projection, dendrite.
Protein Families	Inositol 1,4,5-trisphosphate 5-phosphatase type I family
Database References	HGNC: 6076 OMIM: 600106 KEGG: hsa:3632 STRING: 9606.ENSP00000357583 UniGene: Hs.523360
Tissue Specificity	Predominantly expressed in heart, brain, and skeletal muscle. In brain; high level in Purkinje cells.

Gene Functions References

the overexpression of miR-181a-5p enhanced cell proliferation and invasion and inhibited apoptosis of cervical cancer cells by negatively targeting INPP5A PMID: 28653606
The ataxic phenotype and characteristics neurodegeneration of the Inpp5a Gt(OST50073)Lex mouse indicate a crucial role for Inpp5a in purkinje cells survival. PMID: 26051944
NPP5Amay play a role in development and progression of cutaneous squamous cell carcinoma tumors PMID: 20876729

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.