Recombinant Human EphB1 Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-1825
Recombinant Human EphB1 Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-1825
Catalog No.: BLPSN-1825
Product Overview
| Tag | His |
| Host Species | Human |
| Accession | P54762 |
| Synonym | ELK, EPHT2, Hek6, NET |
| Background | Ephrin type-B receptor 1, also known as EphB1, belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family which 16 known receptors (14 found in mammals) are involved: EPHA1, EPHA2, EPHA3, EPHA4, EPHA5, EPHA6, EPHA7, EPHA8, EPHA9, EPHA1, EPHB1, EPHB2, EPHB3, EPHB4, EPHB5, EPHB6. EphB2 receptor tyrosine kinase phosphorylates syndecan-2 and that this phosphorylation event is crucial for syndecan-2 clustering and spine formation. The Eph family of receptor tyrosine kinases (comprising EphA and EphB receptors) has been implicated in synapse formation and the regulation of synaptic function and plasticity6. Ephrin receptors are components of cell signalling pathways involved in animal growth and development, forming the largest sub-family of receptor tyrosine kinases (RTKs). Ligand-mediated activation of Ephs induce various important downstream effects and Eph receptors have been studied for their potential roles in the development of cancer. EphB receptor tyrosine kinases are enriched at synapses, suggesting that these receptors play a role in synapse formation or function. We find that EphrinB binding to EphB induces a direct interaction of EphB with NMDA-type glutamate receptors. This interaction occurs at the cell surface and is mediated by the extracellular regions of the two receptors, but does not require the kinase activity of EphB. |
| Description | A DNA sequence encoding the human EPHB1 (P54762-1) extracellular domain (Met 1-Pro 540) was expressed, with a His tag at the C-terminus. |
| Source | HEK293 |
| Predicted N Terminal | Met 18 |
| AA Sequence | Met 1-Pro 540 |
| Molecular Weight | The recombinant human EPHB1 consists of 534 a.a. and has a calculated molecular mass of 60 kDa as estimated in SDS-PAGE under reducing conditions. |
| Purity | >95% as determined by SDS-PAGE |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method |
| Bioactivity | Measured by its binding ability in a functional ELISA.1. Immobilized human EPHB1-His at 10 ug/ml (100 ul/well) can bind human EFNB1-Fc2h with a linear ranger of 3.125-200 ng/mL.2. Immobilized human EPHB1-His at 10 ug/ml (100 ul/well) can bind human EFNB2-Fch with a linear ranger of 0.3125-20 ng/mL. |
| Formulation | Lyophilized from sterile PBS, pH 7.4, 5% glycerol. |
| Stability | The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. |
| Usage | For Research Use Only |
| Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
