Recombinant Human EphA7 Protein (His & GST Tag)
Beta LifeScience
SKU/CAT #: BLPSN-1818
Recombinant Human EphA7 Protein (His & GST Tag)
Beta LifeScience
SKU/CAT #: BLPSN-1818
Collections: Enzymes, Kinase, Recombinant proteins
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Product Overview
Tag | His&GST |
Host Species | Human |
Accession | NP_004431 |
Synonym | EHK-3, EHK3, EK11, EPHA7, HEK11 |
Background | Ephrin type-A receptor 7, also known as EphA7, belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family which 16 known receptors (14 found in mammals) are involved: EPHA1, EPHA2, EPHA3, EPHA4, EPHA5, EPHA6, EPHA7, EPHA8, EPHA9, EPHA1, EPHB1, EPHB2, EPHB3, EPHB4, EPHB5, EPHB6. The Eph family of receptor tyrosine kinases (comprising EphA and EphB receptors) has been implicated in synapse formation and the regulation of synaptic function and plasticity6. Eph receptor-mediated signaling, which is triggered by ephrins7, probably modifies the properties of synapses during synaptic activation and remodeling. Ephrin receptors are components of cell signalling pathways involved in animal growth and development, forming the largest sub-family of receptor tyrosine kinases (RTKs). Ligand-mediated activation of Ephs induce various important downstream effects and Eph receptors have been studied for their potential roles in the development of cancer. Down-regulation of EphA7 secondary to hypermethylation has been reported in colorectal cancer. The expression of EphA7 was reduced in all tested gastric cancer cell lines; however, there is marked variability in expression among gastric carcinoma specimens. EphA7 may have roles in the pathogenesis and development of gastric carcinomas. |
Description | A DNA sequence encoding the human EPHA7 (NP_004431) (Gly579-Val998) was fused with the N-terminal His-tagged GST tag at the N-terminus. |
Source | Baculovirus-Insect Cells |
Predicted N Terminal | Met |
AA Sequence | Gly579-Val998 |
Molecular Weight | The recombinant human EPHA7 /GST chimera consists of 657 a.a. and has a calculated molecular mass of 75.2 kDa. The recombinant protein migrates as an approximately 76 kDa band in SDS-PAGE under reducing conditions. |
Purity | >94% as determined by SDS-PAGE |
Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method |
Bioactivity | The specific activity was determined to be 9.5 nmol/min/mg using Poly(Glu:Tyr) 4:1 as substrate. |
Formulation | Supplied as sterile 20mM Tris, 500mM NaCl, pH 8.0, 10% gly. |
Stability | The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. |
Usage | For Research Use Only |
Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |