Recombinant Human EphA2 Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-1798
Recombinant Human EphA2 Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-1798
Catalog No.: BLPSN-1798
Product Overview
| Tag | His |
| Host Species | Human |
| Accession | P29317 |
| Synonym | ARCC2, CTPA, CTPP1, CTRCT6, ECK |
| Background | Eph receptor A2 (Ephrin type-A receptor 2 or EphA2) is a member of the ephrin receptor subfamily of the protein-tyrosine kinase family. The Eph receptors' corresponding family of ligands are the ephrins anchored to cell surfaces. The ephrins and Eph receptors are implicated as positional labels that may guide the development of neural topographic maps. They have also been found implicated in embryonic patterning, neuronal targeting, vascular development and adult neovascularization. The large family of ligands and receptors may make a major contribution to the accurate spatial patterning of connections and cell position in the nervous system. Furthermore, elevated expression of Eph receptors and ephrin ligands is associated with tumors and associated tumor vasculature, suggesting the Eph receptors and ephrin ligands also play critical roles in tumor angiogenesis and tumor growth. Unlike most Eph kinases, which are primarily expressed during development, EphA2 is primarily found in adult human epithelial cells. The cellular functions of EphA2 may be regulating cell growth, survival, migration, and angiogenesis.Unlike other receptor tyrosine kinases, ligand binding is not necessary for EphA2. Rather, the ligand appears to regulate EphA2 subcellular localization and its interactions with downstream adapter and signaling proteins. Eph receptor A2(EphA2) has been demonstrated to critically regulate tumor cell growth, migration and invasiveness. Eph receptor A2(EphA2) is frequently overexpressed and functionally altered in aggressive tumor cells, and that these changes promote metastatic character. |
| Description | A DNA sequence encoding the human EPHA2 (P29317) (Met1-Asn534) was expressed with a His tag at the C-terminus. |
| Source | HEK293 |
| Predicted N Terminal | Ala 24 |
| AA Sequence | Met1-Asn534 |
| Molecular Weight | The recombinant human EPHA2 consists of 522 a.a. and predicts a molecular mass of 57.6 KDa. It migrates as an approximately 65-75 KDa band in SDS-PAGE under reducing conditions. |
| Purity | >95% as determined by SDS-PAGE |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method |
| Bioactivity | Measured by its binding ability in a functional ELISA. Immobilized human EPHA2-His at 10 ug/ml (100 ul/well) can bind human EFNA1-Fch, The EC50 of human EFNA1-Fch is 0.1-0.2 ug/ml. |
| Formulation | Lyophilized from sterile 20mM Tris, 150mM NaCl,- pH 7.5.. |
| Stability | The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. |
| Usage | For Research Use Only |
| Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
