Recombinant Human EphA1 Protein (Fc Tag)
Beta LifeScience
SKU/CAT #: BLPSN-1795
Recombinant Human EphA1 Protein (Fc Tag)
Beta LifeScience
SKU/CAT #: BLPSN-1795
Catalog No.: BLPSN-1795
Product Overview
| Tag | Fc |
| Host Species | Human |
| Synonym | EPH, EPHT, EPHT1 |
| Background | EPHA1 or EPH receptor A1 belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. An important role of Eph receptors and their ligands ephrins is to mediate cell-contact-dependent repulsion. Eph receptors and ephrins also act at boundaries to channel neuronal growth cones along specific pathways, restrict the migration of neural crest cells, and via bidirectional signaling prevent intermingling between hindbrain segments. Eph receptors and ephrins can also trigger an adhesive response of endothelial cells and are required for the remodeling of blood vessels. Eph receptors and ephrins have emerged as key regulators of the repulsion and adhesion of cells that underlie the establishment, maintainence, and remodeling of patterns of cellular organization. The ephrins and Eph receptors are implicated as positional labels that may guide the development of neural topographic maps. |
| Description | A DNA sequence encoding the human EPHA1 (EAL23789.1) (Met1-Glu547) was expressed with the Fc region of human IgG1 at the C-terminus. |
| Source | HEK293 |
| Predicted N Terminal | Lys 26 |
| AA Sequence | Met1-Glu547 |
| Molecular Weight | The recombinant human EPHA1 consists 760 a.a. and predicts a molecular mass of 83.3 kDa. |
| Purity | >90% as determined by SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg protein as determined by the LAL method. |
| Bioactivity | Measured by its binding ability in a functional ELISA.Immobilized human EFNA1-His at 10 ug/ml (100 ul /well) can bind human EPHA1-Fc, The EC50 of human EPHA1-Fc is 10-30 ng/ml. |
| Formulation | Lyophilized from sterile PBS, pH 7.4.. |
| Stability | The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. |
| Usage | For Research Use Only |
| Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
