Recombinant Human DDR2 Protein (Fc Tag)
Beta LifeScience
SKU/CAT #: BLPSN-1575
Recombinant Human DDR2 Protein (Fc Tag)
Beta LifeScience
SKU/CAT #: BLPSN-1575
Catalog No.: BLPSN-1575
Product Overview
| Tag | Fc |
| Host Species | Human |
| Accession | NP_001014796.1 |
| Synonym | CD167, MIG20a, NTRKR3, TKT, TYRO10 |
| Background | Discoidin domain receptor 2 (DDR2) or CD167b (cluster of differentiation 167b) is a kind of protein tyrosine kinases associated with cell proliferation and tumor metastasis, and collagen, identified as a ligand for DDR2, up-regulates matrix metallloproteinase 1 (MMP-1) and MMP-2 expression in cellular matrix. DDR2/CD167b was found to recognise the triple-helical region of collagen X as well as the NC1 domain. Binding to the collagenous region was dependent on the triple-helical conformation. DDR2/CD167b autophosphorylation was induced by the collagen X triple-helical region but not the NC1 domain, indicating that the triple-helical region of collagen X contains a specific DDR2 binding site that is capable of receptor activation. DDR2/CD167b is induced during stellate cell activation and implicate the phosphorylated receptor as a mediator of MMP-2 release and growth stimulation in response to type I collagen. Moreover, type I collagen-dependent upregulation of DDR2/CD167b expression establishes a positive feedback loop in activated stellate cells, leading to further proliferation and enhanced invasive activity.Immune CheckpointImmunotherapyCancer ImmunotherapyTargeted Therapy |
| Description | A DNA sequence encoding the extracellular domain (Met 1-Arg 399) of human DDR2 precursor (NP_001014796.1) was expressed with the fused Fc region of human IgG1 at the C-terminus. |
| Source | HEK293 |
| Predicted N Terminal | Lys 22 |
| AA Sequence | Met 1-Arg 399 |
| Molecular Weight | The recombinant human DDR2/Fc is a disulfide-linked homodimeric protein. The reduced monomer consists of 616 a.a. and has a calculated molecular mass of 69.4 kDa. Due to glycosylation, rhDDR2/Fc monomer migrates as an approximately 87 kDa protein in SDS-PAGE under reducing conditions. |
| Purity | >95% as determined by SDS-PAGE |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method |
| Bioactivity | Measured by its binding ability in a functional ELISA. Immobilized Rat tail Collagen I at 10 ug/ml can bind recombinant human DDR2-Fc Chimera with a linear range of 2.5-80 ng/ml. Scatchard analysis showed the affinity constant (Kd) of recombinant human DDR2-Fc Chimera bound to rat tail collagen I was 6.8 nM. |
| Formulation | Lyophilized from sterile PBS, pH 7.4. |
| Stability | The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. |
| Usage | For Research Use Only |
| Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
