Recombinant HPV18 L1 Protein

Name: Recombinant HPV18 L1 Protein
Brand: Beta LifeScience
SKU: BLA-11484P
Availability: InStock

Collections: Featured viral antigens molecules, Hpv, Recombinant proteins, Viral antigen

Our products are highly customizable to meet your specific needs. You can choose options such as endotoxin removal, liquid or lyophilized forms, preferred tags, and the desired functional sequence range for proteins. Submitting a written inquiry expedites the quoting process.

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Product Overview

Accession	P06794
Synonym	L1 HPV18 major capsid protein L1 Human papilloma virus type 18 major capsid protein L1 Human papillomavirus type 18 L1 Human papillomavirus type 18 major capsid protein L1 Major capsid protein L1
Description	Recombinant HPV18 L1 Protein was expressed in E.coli. It is a Protein fragment
Source	E.coli
AA Sequence	VDVYLPPPSV ARVVNTDDYV TPTSIFYHAG SSRLLTVGNP YFRVPAGGGN KQDIPKVSAY QYRVFRVQLPDPNKFGLPDT SIYNPETQRL VWACAGVEIG RGQPLGVGLS GHPFYNKLDD TESSHAATSN VSEDVRDNVSVDYKQTQLCI LGCAPAIGEH WAKGTACKSR PLSQGDCPPL ELKNTVLEDG DMVDTGYGAM DFSTLQDTKCEVPLDICQSI CKYPDYLQMS ADPYGDSMFF CLRREQLFARHFWNRAGTMG DTVPQSLYIK GTGMPASPGS CVYSPSPSGS IVTSDSQLFN KPYWLHKAQG HNNGVCWHNQLFVTVVDTTP STNLTICAST QSPVPGQYDA TKFKQYSRHVEEYDLQFIFQ LCTITLTADV MSYIHSMNSS ILEDWNFGVPPPPTTSLVDT YRFVQSVAIT CQKDAAPAEN KDPYDKLKFWNVDLKEKFSL DLDQYPLGRK FLVQAGLRRK PTIGPRKRSAPSATTSSKPA
Molecular Weight	83 kDa including tags
Purity	Greater than 95% SDS-PAGE
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Formulation	Liquid Solution
Stability	The recombinant protein samples are stable for up to 12 months at -80°C
Reconstitution	See related COA
Unit Definition	For Research Use Only
Storage Buffer	Shipped at 4°C. Store at -20°C. Avoid freeze / thaw cycle.

Target Details

Target Function	Forms an icosahedral capsid with a T=7 symmetry and a 50 nm diameter. The capsid is composed of 72 pentamers linked to each other by disulfide bonds and associated with L2 proteins. Binds to heparan sulfate proteoglycans on cell surface of basal layer keratinocytes to provide initial virion attachment. This binding mediates a conformational change in the virus capsid that facilitates efficient infection. The virion enters the host cell via endocytosis. During virus trafficking, L1 protein dissociates from the viral DNA and the genomic DNA is released to the host nucleus. The virion assembly takes place within the cell nucleus. Encapsulates the genomic DNA together with protein L2.
Subcellular Location	Virion. Host nucleus.
Protein Families	Papillomaviridae L1 protein family
Database References	KEGG: vg:1489090

Gene Functions References

L1 sequences were strongly methylated in all cervical carcinomas studied; hypothesize that L1 is preferentially methylated after integration of the HPV genome into the cellular DNA PMID: 16472835

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.