Rabbit anti-Human ATP6V0A1 Polyclonal Antibody

Name: Rabbit anti-Human ATP6V0A1 Polyclonal Antibody
Brand: Beta LifeScience
SKU: BLC-00969A
Price: 299.0 USD
Availability: InStock

Immunohistochemistry of paraffin-embedded human kidney tissue using ATP6V0A1 Antibody at dilution of 1:100

Immunofluorescent analysis of A549 cells using ATP6V0A1 Antibody at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Collections: Advance your research with beta lifescience – summer deals on proteins & reagents, Buy antibodies for advanced research

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Product Overview

Product Title	Rabbit anti-Human ATP6V0A1 Polyclonal Antibody
Description	The antibody against ATP6V0A1 was raised in rabbit using the Recombinant Human V-type proton ATPase 116 kDa subunit a isoform 1 protein (80-260AA) as the immunogen. This antibody exists as a non-conjugated isotype IgG, purified by protein G with a purity greater than 95%. This antibody has been validated on ELISA, WB, IHC, IF.
Uniprot Id - Alink	Q93050
Host Species	Rabbit
Reactivity	Human, Mouse, Rat
Target Name	ATP6V0A1
Target Synonyms	a1 antibody; ATP6N1 antibody; ATP6N1A antibody; ATP6V0A1 antibody; ATPase H+ transporting lysosomal (vacuolar proton pump) non catalytic accessory protein 1A (110/116kD) antibody; ATPase H+ transporting lysosomal non catalytic accessory protein 1 (110/116kD) antibody; ATPase H+ transporting lysosomal V0 subunit a1 antibody; ATPase H+ transporting V0 subunit a1 antibody; ATPase, H+ transporting, lysosomal, noncatalytic accessory protein 1A antibody; Clathrin coated vesicle/synaptic vesicle proton pump 116 kDa subunit antibody; Clathrin-coated vesicle/synaptic vesicle proton pump 116 kDa subunit antibody; DKFZp781J1951 antibody; H(+) transporting two sector ATPase 116 kDa accessory protein A1 antibody; Stv1 antibody; V ATPase 116 kDa antibody; V ATPase 116 kDa isoform a1 antibody; V type proton ATPase 116 kDa subunit a antibody; V type proton ATPase 116 kDa subunit a isoform 1 antibody; V-ATPase 116 kDa isoform a1 antibody; V-type proton ATPase 116 kDa subunit a isoform 1 antibody; Vacuolar adenosine triphosphatase subunit Ac116 antibody; Vacuolar proton pump subunit 1 antibody; Vacuolar proton pump, subunit 1 antibody; Vacuolar proton translocating ATPase 116 kDa subunit A antibody; Vacuolar proton translocating ATPase 116 kDa subunit a isoform 1 antibody; Vacuolar type H(+) ATPase 115 kDa subunit antibody; Vph1 antibody; VPP1 antibody; VPP1_HUMAN antibody
Immunogen Description	Recombinant Human V-type proton ATPase 116 kDa subunit a isoform 1 protein (80-260AA)
Immunogen Species	Human
Immunogen Sequence	Complete sequences for the immunogen, target protein, and peptides are available upon request.
Conjugate	Non-conjugated
Clonality	Polyclonal
Isotype	IgG
Purification Method	>95%, Protein G purified
Buffer	0.03% Proclin 300Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form	Liquid
Application	ELISA, WB, IHC, IF
Storage Conditions	Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.