Recombinant Interleukin-4 (IL-4) Proteins

Recombinant human IL-4 is a key tool in modern immunology and inflammation-related research. Known for its broad influence on immune responses, recombinant IL-4 plays an essential role in studies focused on allergy, autoimmune diseases, asthma, and tissue repair. At Beta LifeScience, we offer highly purified and biologically active interleukin 4 recombinant human IL-4 proteins produced through advanced expression systems to ensure consistency and reproducibility in every experiment.

Whether you’re developing biologics, studying cytokine signaling pathways, or screening drug candidates, our human recombinant IL-4 proteins provide the precision and performance trusted by scientists worldwide.

The Role of IL-4 in the Immune System

Interleukin-4 is a multifunctional cytokine that acts as a key mediator of immune regulation. It is primarily secreted by activated T helper 2 (Th2) cells, mast cells, eosinophils, and basophils. IL-4 is crucial in promoting the differentiation of naïve T cells into Th2 cells while suppressing Th1 differentiation. This cytokine is also known to support B cell proliferation and enhance immunoglobulin class switching to IgE and IgG1, which are central to allergic reactions and antibody-mediated immunity.

Because of its central role in immune signaling, interleukin 4 is often implicated in chronic inflammatory conditions, including asthma, dermatitis, and other hypersensitivity disorders. Recombinant human IL-4 allows researchers to explore these pathways in greater depth with controlled dosing and reliable purity.

Why Use Recombinant IL-4?

Using recombinant IL-4 instead of endogenous sources provides unmatched control and reproducibility in experimental settings. Human recombinant IL-4 proteins are engineered to mirror the natural molecule while eliminating contaminants and variability associated with cell extracts or serum.

Key benefits include:

• High purity for sensitive applications
  
• Lot-to-lot consistency to reduce experimental variability
  
• Defined biological activity validated through standardized assays
  
• Compatibility with cell-based, in vivo, and biochemical assays
  

Researchers can confidently explore IL-4’s effects in a wide range of immunological and inflammatory models using Beta LifeScience’s recombinant human IL-4 products.

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Species Variants Available

While recombinant human IL-4 is the most in-demand form, Beta LifeScience also provides interleukin 4 proteins from other species to support comparative studies and animal models. These include:

Featured Products

Cat. No.	Species	Product Description	Host	Structure	Purity	Activity	Features
BLC-05423P	Human	Human IL-4 Protein, Active	Mammalian cell	P05112 (25-153aa)	≥ 95%	ED50: < 0.2 ng/mL	SDS-PAGE, Cell Based Assay
BLC-05428P	Human	Human IL-4 Protein (His), Active	Mammalian cell	P05112 (25-153aa)	≥ 95%	ED50 < 0.2 ng/mL	SDS-PAGE, Cell Based Assay
BL-2119NP	Human	Human IL-4 Protein, Active	Mammalian cell	P05112 (25-153aa)	≥ 95%	ED50: 0.01-0.05 ng/mL	SDS-PAGE, Cell Based Assay, BLI
	Human	Human IL-4 Protein, Active	Mammalian cell	P05112 (25-153aa)	≥ 95%	ED50 ≤ 0.2 ng/mL	SDS-PAGE, Cell Based Assay
BL-2129NP	Human	Human IL-4 Protein (C-6His), Active	Mammalian cell	P05112 (25-153aa)	≥ 95%	ED50: 0.05-0.5 ng/mL	SDS-PAGE, HPLC, Cell Based Assay, BLI
BL-2092NP	Human	Human IL-4 Protein (C-Fc), Active	Mammalian cell	P05112 (25-153aa)	≥ 95%	BLI: affinity constant of <10-3 nM	SDS-PAGE, HPLC, ELISA, BLI
BLC-05400P	Human	Human IL-4 Protein, Active	E.coli	P05112 (25-153aa)	≥ 95%	ED50: < 2 ng/mL	SDS-PAGE, Cell Based Assay
BLC-05413P	Human	Human IL-4 Protein, Active, GMP	E.Coli	P05112 (25-153aa)	≥ 98%	ED50: < 0.2 ng/mL	SDS-PAGE, HPLC, Cell Based Assay
BLC-05335P	Human	Human IL-4 Protein, Active	E.coli	P05112 (25-153aa)	≥ 95%	ED50: < 2 ng/mL	SDS-PAGE, Cell Based Assay
BL-1786NP	Human	Human IL-4 Protein (E. coli), Active	E.coli	P05112 (25-153aa)	≥ 95%	ED50: 10-70 pg/mL	SDS-PAGE, HPLC, Cell Based Assay, BLI
BLC-08618P	Human	Human IL-4 Protein (His-SUMO)	E.coli	P05112 (25-153aa)	≥ 90%	Not Test	SDS-PAGE
BLC-08397P	Human	Human IL-4 Protein (His)	E.coli	P05112 (25-153aa)	≥ 90%	Not Test	SDS-PAGE
BLC-04642P	Human	Human IL-4 Protein (His)	Yeast	P05112 (25-153aa)	≥ 90%	Not Test	SDS-PAGE
BL-0279NP	Mouse	Mouse IL-4 Protein (C-6His), Active	Mammalian cell	P07750 (21-140aa)	≥ 95%	ED50: 0.035 ng/mL	SDS-PAGE, Cell Based Assay
BLC-05312P	Mouse	Mouse IL-4 Protein, Active	E.coli	P07750 (23-140aa)	≥ 95%	ED50: 0.01 ng/mL	SDS-PAGE, Cell Based Assay
BLC-05365P	Mouse	Mouse IL-4 Protein, Active	E.coli	P07750 (23-140aa)	≥ 95%	ED50: 0.01 ng/mL	SDS-PAGE, Cell Based Assay
BLPSN-2903	Mouse	Mouse IL4 Protein, Active	E. coli	P07750 (23-140)	≥ 90%	ED50: 0.4-4 ng/mL	SDS-PAGE, Cell Based Assay
BLPSN-2904	Mouse	Mouse IL4 Protein (Q136D, Y139D, His Tag), Active	Baculovirus	P07750 (1-140aa, Q136D, Y139D)	≥ 95%	ED50: 2-10 ng/mL	SDS-PAGE
BLC-05310P	Mouse	Mouse IL-4 Protein (His), Active	Mammalian cell	P07750 (21-140aa)	≥ 95%	ED50: 0.035 ng/mL	SDS-PAGE, Cell Based Assay
BLC-09079P	Cynomolgus Monkey	Cynomolgus Monkey IL-4 Protein (His)	E.coli	P79339 (25-153aa)	≥ 85%	Not Test	SDS-PAGE
BLC-03290P	Cynomolgus Monkey	Cynomolgus Monkey IL-4 Protein (His)	Yeast	P79339 (25-153aa)	≥ 90%	Not Test	SDS-PAGE
BLC-05383P	Rhesus Macaque	Rhesus Macaque IL-4 Protein, Active	E.Coli	P51492 (25-153aa)	≥ 96%	ED50: < 1.0 ng/mL	SDS-PAGE, Cell Based Assay
BLC-00873P	Llama	Lama Glama IL-4 Protein (His)	Yeast	Q865X5 (25-133aa)	≥ 85%	Not Test	SDS-PAGE
BLC-06937P	Llama	Lama Glama IL-4 Protein (His)	E.coli	Q865X5 (25-133aa)	≥ 90%	Not Test	SDS-PAGE
BLC-00180P	Golden hamster	Golden hamster IL-4 Protein (His&StrepII)	E.coli	A0A1U7QAD7 (25-147aa)	≥ 85%	Not Test	SDS-PAGE
BLC-00528P	Golden Hamster	Mesocricetus Auratus IL-4 Protein (His)	Yeast	Q60440 (20-147aa)	≥ 90%	Not Test	SDS-PAGE
BLC-02864P	Sheep	Sheep IL-4 Protein (His-B2M&Myc)	E.coli	P30368 (25-135aa)	≥ 90%	Not Test	SDS-PAGE
BLC-04678P	Sheep	Sheep IL-4 Protein (His)	Yeast	P30368 (25-135aa)	≥ 90%	Not Test	SDS-PAGE
BLPSN-2899	Canine	Canine IL4 Protein, Active	E. coli	O77762 (25-132aa)	≥ 96%	ED50: < 5-30 ng/mL	SDS-PAGE, Cell Based Assay
BLC-10708P	Dog	Dog IL-4 Protein (His)	E.coli	O77762 (25-132aa)	≥ 90%	Not Test	SDS-PAGE

Applications of Human Recombinant IL-4

The biological actions of interleukin 4 recombinant human IL-4 make it a versatile cytokine used in both basic and translational research. Some of the most common applications include:

• Immunotherapy development: IL-4 plays a role in anti-tumor immunity and has been evaluated for cancer immunotherapy approaches, especially in combination with other cytokines.
• Allergy and asthma models: Since IL-4 promotes IgE synthesis, it's heavily studied in allergic reaction models.
• Autoimmune disease research: IL-4 is studied for its regulatory effects on inflammatory responses in autoimmune diseases like lupus and multiple sclerosis.
• Stem cell differentiation: Human recombinant IL-4 can modulate stem cell fate, influencing macrophage polarization or dendritic cell development.
• T-cell signaling studies: Researchers use IL-4 to investigate pathways related to Th2 activation and function.

Choosing the Right IL-4 for Your Research

Beta LifeScience offers a wide selection of recombinant IL-4 proteins, produced in different host systems such as mammalian cells, E. coli, yeast, and insect cells. Each variant is selected for optimal compatibility with different applications:

• Mammalian cell-derived IL-4 is ideal for researchers requiring native-like post-translational modifications and high bioactivity.
• Bacterial expression IL-4 offers a cost-effective option for routine assays and high-throughput screening.
• Tagged IL-4 versions (e.g., His-tag, Fc-tag) allow for easy purification and detection during assays.

Regardless of the host system, every recombinant IL-4 product undergoes rigorous quality control to ensure proper folding, purity, and activity.

Advantages of Beta LifeScience’s Recombinant IL-4

We understand the importance of reliability in scientific research. That’s why our recombinant IL-4 proteins are manufactured under stringent quality systems, designed to meet the demands of advanced biomedical research. Here’s what sets our IL-4 proteins apart:

• Purity ≥95% or higher verified by SDS-PAGE and HPLC
• Low endotoxin levels suitable for sensitive cell cultures
• Batch-specific biological activity testing to ensure consistent performance
• Flexible formats (with or without tags) for diverse assay requirements
• Support from experienced scientists for protocol optimization and troubleshooting

Whether you're studying basic cytokine biology or working on translational drug development, Beta LifeScience delivers IL-4 you can trust.

Understanding the Mechanism of IL-4

Recombinant IL-4 binds to the IL-4 receptor complex, which consists of IL-4Rα and the common gamma chain (γc). This interaction activates the JAK-STAT signaling pathway, particularly STAT6, which is responsible for driving the transcription of IL-4 responsive genes.

IL-4 signaling leads to:

• Increased MHC class II expression
• Upregulation of CD23 on B cells
• Enhanced IgE class switching
• Suppression of pro-inflammatory cytokines
  

Understanding these mechanisms helps researchers unlock how IL-4 can be both protective and pathological, depending on the biological context.

Integrating IL-4 in Experimental Workflows

For researchers planning to add human recombinant IL-4 to their workflows, here are a few practical tips:

• Titration is key: Always optimize the IL-4 concentration for your specific cell type and assay conditions. Effective concentrations vary widely depending on the application.
• Storage recommendations: Store IL-4 proteins at –80°C in small aliquots to avoid repeated freeze-thaw cycles. Lyophilized versions offer greater stability and ease of use.
• Combine with other cytokines: IL-4 often works in synergy with IL-13, IL-10, or GM-CSF in polarization assays, co-culture systems, or cytokine cocktails.

For more detailed experimental recommendations, our team is available to assist you.

Trusted by Scientists Worldwide

Beta LifeScience is proud to support cutting-edge research by offering dependable recombinant cytokines, including recombinant IL-4. Our customers include leading academic institutions, pharmaceutical companies, and biotech firms. We are committed to delivering proteins that meet the highest standards, enabling faster discoveries and reliable results.

Whether you're studying IL-4’s role in adaptive immunity or developing novel therapeutics, you can count on our recombinant human IL-4 proteins for consistency, purity, and performance.

FAQs

What is recombinant human IL-4?

Recombinant human IL-4 is a lab-produced form of the interleukin-4 protein, used to study immune responses, allergic conditions, and therapeutic development.

What are the applications of recombinant IL-4 proteins?

Recombinant IL-4 is used in immunology research, drug screening, cell culture experiments, and inflammation studies to evaluate cytokine signaling.

Are your IL-4 proteins bioactive?

Yes, our recombinant IL-4 proteins are tested for biological activity through cell-based assays, ELISA, and BLI to ensure consistent and reliable results.

What species are available for IL-4 proteins?

We offer IL-4 proteins from human, mouse, rhesus macaque, cynomolgus monkey, dog, sheep, and more to support a wide range of research needs.

How should I store recombinant IL-4 proteins?

Store the proteins at –20°C or –80°C, as specified in the datasheet, and avoid repeated freeze-thaw cycles to maintain stability.

Can I use human recombinant IL-4 for in vivo studies?

While many products are bioactive, always consult the datasheet and regulatory guidelines before using recombinant IL-4 in animal models or therapeutic research.