Recombinant Mouse A Disintegrin And Metalloproteinase With Thrombospondin Motifs 5 (ADAMTS5) Protein (His)
Beta LifeScience
SKU/CAT #: BLC-03697P
Greater than 90% as determined by SDS-PAGE.
Recombinant Mouse A Disintegrin And Metalloproteinase With Thrombospondin Motifs 5 (ADAMTS5) Protein (His)
Beta LifeScience
SKU/CAT #: BLC-03697P
Collections: Enzymes, Featured enzyme molecules, Protease, Recombinant proteins
Our products are highly customizable to meet your specific needs. You can choose options such as endotoxin removal, liquid or lyophilized forms, preferred tags, and the desired functional sequence range for proteins. Submitting a written inquiry expedites the quoting process.
Product Overview
Description | Recombinant Mouse A Disintegrin And Metalloproteinase With Thrombospondin Motifs 5 (ADAMTS5) Protein (His) is produced by our E.coli expression system. This is a full length protein. |
Purity | Greater than 90% as determined by SDS-PAGE. |
Uniprotkb | Q9R001 |
Target Symbol | ADAMTS5 |
Synonyms | Adamts5; A disintegrin and metalloproteinase with thrombospondin motifs 5; ADAM-TS 5; ADAM-TS5; ADAMTS-5; EC 3.4.24.-; ADMP-2; Aggrecanase-2; Implantin |
Species | Mus musculus (Mouse) |
Expression System | E.coli |
Tag | N-6His |
Target Protein Sequence | SISRARQVELLLVADSSMARMYGRGLQHYLLTLASIANRLYSHASIENHIRLAVVKVVVLTDKDTSLEVSKNAATTLKNFCKWQHQHNQLGDDHEEHYDAAILFTREDLCGHHSCDTLGMADVGTICSPERSCAVIEDDGLHAAFTVAHEIGHLLGLSHDDSKFCEENFGTTEDKRLMSSILTSIDASKPWSKCTSATITEFLDDGHGNCLLDLPRKQILGPEELPGQTYDATQQCNLTFGPEYSVCPGMDVCARLWCAVVRQGQMVCLTKKLPAVEGTPCGKGRVCLQGKCVDKTKKKYYSTSSHGNWGSWGPWGQCSRSCGGGVQFAYRHCNNPAPRNSGRYCTGKRAIYRSCSVTPCPPNGKSFRHEQCEAKNGYQSDAKGVKTFVEWVPKYAGVLPADVCKLTCRAKGTGYYVVFSPKVTDGTECRPYSNSVCVRGRCVRTGCDGIIGSKLQYDKCGVCGGDNSSCTKIIGTFNKKSKGYTDVVRIPEGATHIKVRQFKAKDQTRFTAYLALKKKTGEYLINGKYMISTSETIIDINGTVMNYSGWSHRDDFLHGMGYSATKEILIVQILATDPTKALDVRYSFFVPKKTTQKVNSVISHGSNKVGPHSTQLQWVTGPWLACSRTCDTGWHTRTVQCQDGNRKLAKGCLLSQRPSAFKQCLLKKC |
Expression Range | 262-930aa |
Protein Length | Full Length of Mature Protein |
Mol. Weight | 77.8kDa |
Research Area | Signal Transduction |
Form | Liquid or Lyophilized powder |
Buffer | Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. |
Reconstitution | Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%. |
Storage | 1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C. |
Notes | Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week. |
Target Details
Target Function | Metalloproteinase that plays an important role in connective tissue organization, development, inflammation and cell migration. Extracellular matrix (ECM) degrading enzyme that shows proteolytic activity toward the hyalectan group of chondroitin sulfate proteoglycans (CSPGs) including ACAN, VCAN, BCAN and NCAN. Cleavage within the hyalectans occurs at Glu-Xaa recognition motifs. Plays a role in embryonic development, including limb and cardiac morphogenesis, and skeletal muscle development through its VCAN remodeling properties. Cleaves VCAN in the pericellular matrix surrounding myoblasts, facilitating myoblast contact and fusion which is required for skeletal muscle development and regeneration. Participates in the development of brown adipose tissue and browning of white adipose tissue. Plays an important role for T-lymphocyte migration from draining lymph nodes following viral infection. |
Subcellular Location | Secreted, extracellular space, extracellular matrix. |
Database References | |
Tissue Specificity | Expressed in skeletal muscle. |
Gene Functions References
- ADAMTS5 plays a functional role in development of brown adipose tissue and browning of white adipose tissue. PMID: 28702327
- Energy expenditure and thermogenesis were not significantly different between KO and WT ADAMTS5-J mice (in contrast to somewhat enhanced levels in ADAMTS5-P mice). Insulin sensitivity was improved in the ADAMTS5-J KO mice, and they were protected against non-alcoholic steatohepatitis in the DIO model PMID: 29293679
- Adamts5(-/-) mice were protected from hepatic mitochondrial dysfunction, as indicated by increased mitochondrial respiratory chain complex activity, higher ATP levels and higher expression of antioxidant enzymes. Absence of ADAMTS5 preserves liver integrity in a diet-induced obesity model. PMID: 27254774
- research emphasises the importance of ADAMTS5 expression in the control of influenza virus infection and highlights the potential for development of ADAMTS5-based therapeutic strategies to reduce morbidity and mortality PMID: 27855162
- TS5 protein functions to suppress glucose uptake in adipose-derived stromal cells and thereby inhibits the synthesis, and promotes the intracellular degradation of Acan and Vcan by an ADAMTS other than TS5. PMID: 25840345
- Data suggest that ADAMTS-5 oligomerization is required for full aggrecanase activity in vitro and in situ (as seen in knee joint of mouse model of inflammatory arthritis); thus, blocking oligomerization inhibits ADAMTS-5 activity. PMID: 26668318
- aggrecan and brevican proteolysis is compensated in Adamts4-/- or Adamts5-/- mice by ADAMTS proteoglycanase family members but a threshold of versican proteolysis is sensitive to the loss of a single ADAMTS proteoglycanase during spinal cord injury PMID: 25101296
- The present study reveals ADAMT-5 expression by mast cells(MCs) and that MC activation regulates the expression of the protease, thus implicating the ADAMT-5 of protease in MC function. PMID: 23154421
- Western blot analyses indicated that aggrecanase-generated proteoglycan fragments are produced after SCI. PMID: 23562508
- RelA/p65 is a potent transcriptional activator of ADAMTS5 in chondrocytes during osteoarthritis development. PMID: 23963448
- Repair of biomechanically compromised tendons exhibiting midsubstance chondroid accumulation requires ADAMTS5. PMID: 23754494
- this study identified, for the first time, several genes that have an ADAMTS-5-independent role in osteoarthritis(OA), identifying them as possible OA initiation candidates. PMID: 23436205
- The serine protease tissue plasminogen activator (tPA) and two matrix metalloproteinases, ADAMTS-4 and ADAMTS-5, were identified as Reelin cleaving enzymes. PMID: 23082219
- Versican processing by ADAMTS5 and ADAMTS15 contribute to muscle fiber formation. PMID: 23233679
- the first evidence implicating ADAMTS-5 in the regulation of proteoglycan turnover and lipoprotein retention in atherosclerosis. PMID: 22493487
- The role of ADAMTS5 in tendon is to remove pericellular and interfibrillar aggrecan to maintain the molecular architecture responsible for normal tissue function. PMID: 21928430
- Adamts5 induction in joint components other than cartilage, and its post-translational activation by PACE4 and/or furin may be important in the pathophysiology of arthritis. PMID: 21800360
- Matrilin-4 is processed by ADAMTS-5 in late Golgi vesicles present in growth plate chondrocytes of defined differentiation state PMID: 21539915
- a physiological function of ADAMTS5 in dermal fibroblasts is to maintain optimal versican content and PCM volume by continually trimming versican in hyaluronan-versican aggregates. PMID: 21828051
- Altered versican cleavage in ADAMTS5 deficient mice; a novel etiology of myxomatous valve disease PMID: 21749862
- ADAMTS5 ablation did not eliminate aggrecanase activity from the articular cartilage but blocked fibrosis and resulted in the accumulation of aggrecan in the articular cartilage. PMID: 21337391
- extracellular matrix degrading enzyme PMID: 11831030
- ADAMTS1, ADAMTS4, and ADAMTS5 are expressed in patterns that relate to the expression pattern of versican in granulosa cells of small follicles, expanded cumulus cell-oocyte complexes, and endothelial cells of the ovary. PMID: 15659705
- After surgically induced joint instability, there was significant reduction in the severity of cartilage destruction in the ADAMTS5 knockout mice compared with wild-type mice PMID: 15800624
- ADAMTS5 is the major aggrecanase in mouse cartilage, both in vitro and in a mouse model of inflammatory arthritis PMID: 15800625
- ADAMTS-5 is entirely responsible for cleavage in the interglobular domain, but cleavage in the chondroitin sulfate-rich region may be driven by ADAMTS-4 PMID: 17255106
- aggrecan loss with aggrecan processing in mice with single and double deletions of ADAMTS-4 and -5 activity (Deltacat) PMID: 17938173
- Deletion of ADAMTS-4/5 provided significant protection against proteoglycan degradation ex vivo and decreased the severity of murine osteoarthritis PMID: 17968948
- Data show that expression of Adamts5 during neuromuscular development and in smooth muscle cells coincides with the broadly distributed proteoglycan versican, an ADAMTS5 substrate. PMID: 19250981
- Fibroblast growth factor 2 is an intrinsic chondroprotective agent that suppresses ADAMTS-5 and delays cartilage degradation in murine osteoarthritis. PMID: 19565481
- The occurrence of less severe osteoarthritis-like cartilage destruction in both syndecan-4-deficient mice and syndecan-4-specific antibody-treated wild-type mice results from a marked decrease in ADAMTS-5 activity. PMID: 19684582
- show that combinatorial mouse alleles for the secreted metalloproteases Adamts5, Adamts20 (bt), and Adamts9 result in fully penetrant soft-tissue syndactyly. PMID: 19922873