Recombinant Human Transient Receptor Potential Cation Channel Subfamily V Member 3 (TRPV3) Protein (His-Flag)

Name: Recombinant Human Transient Receptor Potential Cation Channel Subfamily V Member 3 (TRPV3) Protein (His-Flag)
Brand: Beta LifeScience
SKU: BLC-02035P
Price: 3672.8 USD
Availability: InStock

Greater than 85% as determined by SDS-PAGE.

Collections: Other recombinant proteins, Recombinant proteins

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Product Overview

Description	Recombinant Human Transient Receptor Potential Cation Channel Subfamily V Member 3 (TRPV3) Protein (His-Flag) is produced by our E.coli expression system. This is a full length protein.
Purity	Greater than 85% as determined by SDS-PAGE.
Uniprotkb	Q8NET8
Target Symbol	TRPV3
Species	Homo sapiens (Human)
Expression System	in vitro E.coli expression system
Tag	N-10His-Flag
Target Protein Sequence	MKAHPKEMVPLMGKRVAAPSGNPAILPEKRPAEITPTKKSAHFFLEIEGFEPNPTVAKTSPPVFSKPMDSNIRQCISGNCDDMDSPQSPQDDVTETPSNPNSPSAQLAKEEQRRKKRRLKKRIFAAVSEGCVEELVELLVELQELCRRRHDEDVPDFLMHKLTASDTGKTCLMKALLNINPNTKEIVRILLAFAEENDILGRFINAEYTEEAYEGQTALNIAIERRQGDIAALLIAAGADVNAHAKGAFFNPKYQHEGFYFGETPLALAACTNQPEIVQLLMEHEQTDITSRDSRGNNILHALVTVAEDFKTQNDFVKRMYDMILLRSGNWELETTRNNDGLTPLQLAAKMGKAEILKYILSREIKEKRLRSLSRKFTDWAYGPVSSSLYDLTNVDTTTDNSVLEITVYNTNIDNRHEMLTLEPLHTLLHMKWKKFAKHMFFLSFCFYFFYNITLTLVSYYRPREEEAIPHPLALTHKMGWLQLLGRMFVLIWAMCISVKEGIAIFLLRPSDLQSILSDAWFHFVFFIQAVLVILSVFLYLFAYKEYLACLVLAMALGWANMLYYTRGFQSMGMYSVMIQKVILHDVLKFLFVYIVFLLGFGVALASLIEKCPKDNKDCSSYGSFSDAVLELFKLTIGLGDLNIQQNSKYPILFLFLLITYVILTFVLLLNMLIALMGETVENVSKESERIWRLQRARTILEFEKMLPEWLRSRFRMGELCKVAEDDFRLCLRINEVKWTEWKTHVSFLNEDPGPVRRTDFNKIQDSSRNNSKTTLNAFEEVEEFPETSV
Expression Range	1-790aa
Protein Length	Full Length
Mol. Weight	93.1 kDa
Research Area	Neuroscience
Form	Liquid or Lyophilized powder
Buffer	Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution	Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	Putative receptor-activated non-selective calcium permeant cation channel. It is activated by innocuous (warm) temperatures and shows an increased response at noxious temperatures greater than 39 degrees Celsius. Activation exhibits an outward rectification. May associate with TRPV1 and may modulate its activity. Is a negative regulator of hair growth and cycling: TRPV3-coupled signaling suppresses keratinocyte proliferation in hair follicles and induces apoptosis and premature hair follicle regression (catagen).
Subcellular Location	Membrane; Multi-pass membrane protein.
Protein Families	Transient receptor (TC 1.A.4) family, TrpV subfamily, TRPV3 sub-subfamily
Database References	HGNC: 18084 OMIM: 607066 KEGG: hsa:162514 STRING: 9606.ENSP00000461518 UniGene: Hs.446255
Associated Diseases	Olmsted syndrome (OLMS); Palmoplantar keratoderma, non-epidermolytic, focal 2 (FNEPPK2)
Tissue Specificity	Abundantly expressed in CNS. Widely expressed at low levels. Detected in dorsal root ganglion (at protein level). Expressed in the keratinocyte layers of the outer root sheath and, to lesser extent, to the matrix of the hair follicles (at protein level).

Gene Functions References

Data suggest that Thr264 in TRPV3 is key ERK1 phosphorylation site mediating EGFR-induced sensitization of TRPV3 to stimulate signaling pathways involved in regulating skin homeostasis. (TRPV3 = transient receptor potential cation channel subfamily V member-3; ERK1 = extracellular signal-regulated kinase-1; EGFR = epidermal growth factor receptor) PMID: 29084846
This study identified a direct regulatory effect of MBTPS2 on TRPV3 which can partially contribute to the overlapping clinical features of IFAP and Olmsted syndromes under a common signaling pathway. PMID: 28717930
TRPV3 may contribute to pruritus in burn scars through TSLP. PMID: 29140280
the expression of transient receptor potential vanilloid-1 (TRPV1), transient receptor potential vanilloid-2 (TRPV2) and transient receptor potential vanilloid-3 (TRPV3) channels in native human odontoblasts, was examined. PMID: 28905239
TRPV3 was highly expressed in the infiltrating eosinophils and mucosal epithelium of the nasal polyps of ECRS, and further that the more severe the refractoriness was after surgery, the higher the TRPV3 expression was in nasal polyps. PMID: 28462829
Data indicate that the restoration of a single residue that is apparently missing in the use-dependent homologs could largely eliminate the use dependence of heat sensitivity of vanilloid receptor transient receptor potential 3 (TRPV3). PMID: 28154143
TRPV3 mutants causing Olmsted Syndrome induce impaired cell adhesion and nonfunctional lysosomes PMID: 27754757
*We describe two cases of Olmsted-like syndrome in a Mongolian family. *The underlying cause was a previously undescribed G573V point mutation in TRPV3. PMID: 27273692
The results of the present study show that polymorphism of TRPV3 contributed towards symptom severity in FM. PMID: 27079220
A novel mutation in TRPV3 gene causes atypical familial Olmsted syndrome in a Chinese family. PMID: 26902751
High Transient receptor potential vanilloid 3 protein expression could promote the proliferation of lung cancer cells. Transient receptor potential vanilloid 3 inhibition decreased [Ca2+]i of lung cancer cells and cell cycle arrest at the G1/S boundary. PMID: 27023518
these data suggest that TRPV3 sparklets cause dilation of cerebral parenchymal arterioles by activating IK and SK channels in the endothelium PMID: 26453324
TRPV3 missense mutation identified as a cause of the rare Olmsted syndrome. PMID: 26067147
TPRV3 was significantly elevated in the epidermis of burn scars with pruritus. PMID: 24695993
Study illustrates the antiadipogenic role of TRPV3 in the adipocytes. PMID: 25774551
this study provides powerful tools to broaden our understanding of ligand interaction with TRPV channels, and the availability of purified human TRPV3 opens up perspectives for further structural and functional studies PMID: 25829496
A mutation in TRPV3 causes focal palmoplantar keratoderma in a Chinese family. PMID: 25285920
Demonstrate similarities but also notable differences in TRPV3 pharmacology between recombinant and native systems. PMID: 23848361
a TRPV3 mutation has a role in Olmsted syndrome [case report] PMID: 24463422
TRPV3 missense mutation found in patient with Olmsted syndrome. PMID: 24452206
TRPV3-ARD with characteristic finger 3 loop likely plays an important role in channel function and pharmacology. PMID: 24248473
TRPV3 has roles in skin physiology and in certain skin diseases [review] PMID: 23800054
The Olmsted syndrome patient was found to harbour a previously undescribed 1718G-C transversion in TRPV3, causing a G573A point mutation with immunological dysregulation function. PMID: 23692804
TRPV3 is a therapeutic target for itch PMID: 22475759
Nucleotide sequencing of five additional affected individuals also revealed missense mutations in TRPV3. PMID: 22405088
Nominal association was confirmed for TRPV3 rs7217270 in migraine with aura and TRPV1 rs222741 in the overall migraine group. PMID: 22162417
It was concluded that the sensitization of TRPV3 is intrinsic to the channel itself and occurs as a result of hysteresis of channel gating. PMID: 22006988
TRPV3 channel is expressed in skin, its likely role is to detect noxious cold temperatures. PMID: 21490957
PI(4,5)P(2)-dependent modulation of TRPV3 activity represents an attractive mechanism for acute regulation of keratinocyte signaling cascades that control cell proliferation and the release of autocrine and paracrine factors. PMID: 21321070
Farnesyl pyrophosphate is the firstly identified endogenous TRPV3 activator that causes nociception PMID: 20395302
A multiligand binding site for ATP and calmodulin previously identified in the TRPV1 ankyrin repeat domain is conserved in TRPV3 and TRPV4, but not TRPV2. PMID: 19864432
member of the vanilloid channel family that is expressed in skin, tongue, dorsal root ganglion, trigeminal ganglion, spinal cord and brain; a calcium-permeable temperature-sensitive cation channel PMID: 12077604
temperature-sensitive (but capsaicin-insensitive) vanilloid receptor-like protein; may represent an additional vanilloid receptor subunit involved in the formation of heteromeric vanilloid receptor channels PMID: 12077606
Ca(2+) inhibits TRPV3 from both the extracellular and intracellular sides. The inhibition is sequentially reduced, appearing as sensitization to repetitive stimulations. PMID: 18178557
vanilloid type 3 (TRPV3) channel is crucially involved in pruritic dermatitis [review] PMID: 19209153

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

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